VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles


Volume: 33, Issue: 1, March 2020

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Page Visits: 74

Doi: 10.1007/s42535-019-00094-5
Doi Link:
First Page: 158
Last Page: 165
Published: 21 January, 2020

Enhanced anti-ageing and wound healing properties of Ficus religiosa L. bark, leaf and aerial root extract in human keratinocytes cell line (HaCaT)


Plants have played a vital role in curative and preventive healthcare. Ficus religiosa L., a medicinally important tree has been used as a traditional medicine to treat various ailments and skin diseases. However, its skin anti-ageing properties are not yet scientifically evaluated. Here we report the comparison of F. religiosa bark (FRAqB), leaf (FRAqL) and aerial-roots (FRAqAR) extracts for skin anti-ageing and wound healing properties. FRAqB, FRAqL and FRAqAR extracts preparation, cell culture, RNA isolation, real time PCR, astringent activity and wound healing assay were carried out using standard methods. Axio Observer FL-microscope and ImageJ software were used for wound healing assay analysis. The real time PCR study reveals that matrix metalloproteinase-1 (MMP) RNA was downregulated to 75% and 95% for FRAqB and FRAqAR, respectively. For procollagen C-endopeptidase enhancer 1 (PCOLCE), FRAqB and FRAqL enhanced RNA levels by 500% and 300%, respectively. FRAqB and FRAqL increased wound healing area to about 60% and FRAqAR by 50%. All three extracts demonstrated astringent activity which is significant for skin tightening. The study proves that extracts of F. religiosa are prominent candidates for pharmaceutical and cosmetic applications such as skin anti-ageing and wound healing.



Anti-ageing, Astringent, Ficus religiosa L., HaCaT, MMP-1, PCOLCE, TERT, Wound healing


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Acknowledgements :

This research was supported by SP1601-001 for Anti-Ageing Research Institute of BIO-FD&C Co., LTD. VA Parasharami would like to thank CSIR-NCL’s in house funding for research on F. religiosa L.

Author Information:

Prem Pandey
Symbiosis Center for Nanoscience and Nanotechnology (SCNN), Symbiosis International University, Pune, India

Hyo Hyun Seo
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Hye-In Kim
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Seung Hwan Ryu
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Medini Dingre
Biochemical Research Division, GGENEX, Pune, India

Sang Hyun Moh
Anti-Ageing Research Institute of BIO-FD&C Co. Ltd., Incheon, Korea

Atul Kulkarni
Symbiosis Center for Nanoscience and Nanotechnology (SCNN), Symbiosis International University, Pune, India
, Varsha A. Parasharami
Plant Tissue Culture Division, National Chemical Laboratory, Pune, India

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