VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 4, December 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 53

Doi: 10.1007/s42535-019-00060-1
Doi Link: https://doi.org/10.1007/s42535-019-00060-1
First Page: 486
Last Page: 493
Published: 11 September, 2019

Mitigation of drought stress in wheat crop by drought tolerant endophytic bacterial isolates


Abstract:

Bacteria with potential to alleviate abiotic stresses in combination with plant growth promotion are extremely useful in sustainable agriculture. The present study involves the isolation and evaluation of 14 bacterial isolates obtained from four upland rice roots in varying concentrations of PEG-6000. Three potential isolates MKA2, MKA3 and MKA4 were selected for further experiments. A pot experiment was conducted with four treatments under well watered and drought conditions. Water deficit treatment was imposed by reducing the amount of water added to 25% of the field capacity. Twenty days post stress results showed that drought stress affected the physiological and biochemical parameters of uninoculated wheat seedlings. Inoculation with the three bacterial isolates diluted the adverse effects of drought on relative water content and membrane stability index, and thus improved the water use efficiency over the uninoculated control. Inoculation also improved the shoot and root biomass of wheat plants in comparison to uninoculated control treatment under drought stress. Increased levels of super oxide dismutase and catalase activities were observed in treated plants as compared to control plants thus protecting the plants from oxidative damage. Thus, these potent bacterial isolates could be used effectively to ameliorate plant water intake efficiency and improve sustainability in wheat crop under drought conditions.

Vegetos

Keywords:


Drought tolerant endophytic bacteria, Ameliorate, Drought stress, Field capacity


References:


  1. Abbasi S, Zahedi H, Sadeghipour O, Akbari R (2013) Effect of plant growth promoting rhizobacteria (PGPR) on physiological parameters and nitrogen content of soybean grown under different irrigation regimes. Res Crops 14:798–803

  2. Aebi H (1974) Catalase in bergmeyer hans ulrich. Methods of enzymatic analysis, 5th edn. Academic Press Incorporated, New York

  3. Ali A, Ali N, Ullah N, Ullah F, Adnan M, Ahmed ZS (2013) Effect of drought stress on the physiology and yield of the Pakistani wheat germplasms. Int J Adv Res Technol 2:419–430

  4. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399

  5. Arau JL, Slafer GA, Royo C, Serret MD (2008) Breeding for yield potential and stress adaptation in cereals. Crit Rev Plant Sci 27:377–412

  6. Armada E, Roldan A, Azcon R (2014) Differential activity of autochthonous bacteria in controlling drought stress in native Lavandula and Salvia plants species under drought conditions in natural arid soil. Microb Ecol 67:410–420

  7. Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) Photoinhibition. Elsevier, Amsterdam, pp 227–287

  8. Ashraf M, Hasnain S, Berge O, Mahmood T (2004) Inoculating wheat seedling with exopolysaccharide producing bacteria restricts sodium uptake and stimulates plant growth under salt stress. Biol Fert Soils 40:157–162

  9. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

  10. Beck EH, Fettig S, Knake C, Hartig K, Bhattarai T (2007) Specific and unspecific responses of plants to cold and drought stress. J Biosci 32:501–510

  11. Claussen W (2005) Proline as a measure of stress in tomato plants. Plant Sci 168:241–248

  12. Creus CM, Sueldo RJ, Barassi CA (2004) Water relations and yield in Azospirillum inoculated wheat exposed to drought in the field. Can J Bot 82:273–281

  13. Dardanelli MS, Fernandez de Cordoba FJ, Rosario Espuny M, Rodriguez Carvajal MA, Soria Diaz ME, Gil Serrano AM, Okon Y, Megias M (2008) Effect of Azospirillum brasilense coinoculated with Rhizobium on Phaseolus vulgaris flavonoids and Nod factor production under salt stress. Soil Biol Biochem 40:2713–2721

  14. Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101

  15. Editorial (2010) How to feed a hungry world. Nature 466:531–532

  16. Farooq M, Wahid A, Kobayashi N, Fujeta D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29:185–212

  17. Glick BR, Liu C, Ghosh S, Dumbroff EB (1997) The effect of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2 on the development of canola seedlings subjected to various stresses. Soil Biol Biochem 29:12331239

  18. Glick BR, Cheng Z, Czarny J, Duan J (2007) Promotion of plant growth by ACC deaminase-producing soil bacteria. In: Bakker PAH, Raaijmakers J, Lemanceau P, Bloemberg G (eds) New perspectives and approaches in plant growth-promoting rhizobacteria research. Springer, Netherlands, pp 329–339

  19. Gou W, Tian L, Ruan Z, Zheng P, Chen F, Zhang L, Cui Z, Zheng P, Li Z, Gao M, Shi W, Zhang L, Liu J, Hu J (2015) Accumulation of choline and glycine betaine and drought stress tolerance induced in maize (Zea mays) by three plant growth promoting rhizobacteria (PGPR) strains. Pak J Bot 47:581–586

  20. Gusain YS, Singh US, Sharma AK (2015) Bacterial mediated amelioration of drought stress in drought tolerant and susceptible cultivars of rice (Oryza sativa L.). Afr J Biotechnol 14:764–773

  21. Kanwal S, Ilyas N, Ahmad B, Batool N, Arshad M (2016) Alleviation of drought stress in wheat by combined application of PGPR, compost and mineral fertilizer in wheat. J Plant Nutr. https://doi.org/10.1080/01904167.2016.1263322

  22. Kohler J (2008) Plant-growth-promoting rhizobacteria and arbuscular mycorrhizal fungi modify alleviation biochemical mechanisms in water-stressed plants. Funct Plant Biol 35:141151

  23. Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell Environ 25:275-24

  24. Mancosu N, Snyder RL, Kyriakakis G, Spano D (2015) Water scarcity and future challenges for food production. Water 7:975–992

  25. Marulanda A, Porcel R, Barea JM, Azco´ n R (2007) Drought tolerance and antioxidant activities in lavender plants colonized by native drought-tolerant or drought-sensitive Glomus species. Microb Ecol 54:543552

  26. Marulanda A, Azco´n R, Ruı´z-Lozano JM, Aroca R (2008) Differential effects of a Bacillus megaterium strain on Lactuca sativa plant growth depending on the origin of the arbuscular mycorrhizal fungus coinoculated: physiologic and biochemical traits. J Plant Growth Regul 27:1018

  27. Marulanda A, Barea JM, Azcón RJ (2009) Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. J Plant Growth Regul 28:115–124

  28. Mayak S, Tirosh T, Glick B (2004) Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem 42:56–572

  29. Miller G, Susuki N, Ciftci-Yilmaz S, Mittler R (2010) Reactive oxygen species homeostasis and signaling during drought and salinity stresses. Plant Cell Environ 33:453–467

  30. Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410

  31. Nadeem SM, Zahir ZA, Naveed M, Arshad M (2007) Preliminary investigations on inducing salt tolerance in maize through inoculation with rhizobacteria containing ACC deaminase activity. Can J Microbiol 53:1141–1149

  32. Okuyama LA, Federizzi LC, Barbosaneto JF (2004) Correlation and path analysis of yield and its components and plant traits in wheat. Ciência Rural 34:1701–1708

  33. Sairam RK (1994) Effect of moisture stress on physiological activities of two contrasting wheat genotypes. Indian J Exp Biol 32:594–597

  34. Sandhya V, SkZ A, Grover M, Reddy G, Venkateswaralu B (2010) Effect of plant growth promoting Pseudomonas spp. on compatible solutes antioxidant status and plant growth of maize under drought stress. Plant Growth Regul 62:21–30

  35. Shintu PV, Jayaram KM (2015) Phosphate solubilizing bacteria (Bacillus polymyxa)—an effective approach to mitigate drought in tomato (Lycopersicon esculentum Mill). Trop Plant Res 2:17–22

  36. Singh RP, Jha PN (2017) The PGPR Stenotrophomonas maltophilia SBP-9 augments resistance against biotic and abiotic stress in wheat plants. Front Microbiol. https://doi.org/10.3389/fmicb.01945

  37. Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175:408–413

  38. Timmusk S, Wagner EGH (1999) The plant growth promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses. Mol Plant-Microb Interact 12:951959

  39. Vieira da Silva J, Naylor AW, Kramer PJ (1974) Some ultrastructural and enzymatic effects of drought stress in cotton (Gossypium hirsutum L.) leaves. Proc Natl Acad Sci 71:3243–3324

  40. Yang J, Kloepper JW, Ryu CM (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci 14:14

  41. Zhang H, Murzello C, Sun Y, Kim X, Mi-S R, Jeter RM, Zak JC, Scot Dowd E, Pare PW (2010) Choline and osmotic-stress tolerance induced in Arabidopsis by the soil microbe Bacillus subtilis (GB03). Mol Plant Microb Interact 23:1097–1104


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Acknowledgements :



This work was partially supported by the project granted by Indian Council of Agricultural Research—Incentivizing Research in Agriculture on Biological Nitrogen fixation ICAR-BNF, IARI. The first author is thankful to Division of Microbiology, ICAR—Indian Agricultural Research Institute, New Delhi, India for research facilities.


Author Information:



Meenakshi
Amity Institute of Microbial Technology, Amity University, Noida, India
molikule.sharma@gmail.com




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