VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 3, September 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 34

Doi: 10.1007/s42535-019-00041-4
Doi Link: https://doi.org/10.1007/s42535-019-00041-4
First Page: 269
Last Page: 274
Published: 20 July, 2019

Improved plant regeneration method of Artocarpus lakoocha Roxb. from immature seeds


Abstract:

An efficient in vitro propagation method is developed for Artocarpus lakoocha Roxb. through direct shoot regeneration from cotyledonary node region of seedlings developed from immature seeds. The immature seeds were germinated on Murashige and Skoog (MS) medium having different concentrations of either 6-benzyl aminopurine (BA) or kinetin or Thidiazuron and subsequently, the cotyledonary node with primary shoot was transferred to MS medium without any growth regulator for shoot multiplication. Maximum (7.23 ± 0.46) shoots regenerated when immature seeds were cultured for 21 days on MS medium having 4.44 µM BA for germination. For rooting, shoots were pulse treated for 48 h with different concentrations of Indole-3-acetic acid (IAA) or Indole-3-butyric acid (IBA) followed by transfer on agar gelled MS basal medium. About 94.45% shoots rooted on pulse treatment with 5.0 µM IBA. Plantlets obtained after rooting were hardened and acclimatized into soil with 85–90% survival. The plantlets established into soil had similar vegetative morphology to the mother plant.

Vegetos

Keywords:


Cotyledonary node, Immature seed, Pulse treatment, Rooting, Shoots


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Acknowledgements :



Financial assistance vide letter no. F. No. 33-468/2007 (SR) by University Grants Commission, New Delhi, India to MS is gratefully acknowledged.


Author Information:



Madhulika Singh
Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, India
msinghup@gmail.com
Saurabh Bhatti
Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, India


Shiv Kumar Verma
Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur, India




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