VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 3, September 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 109

Doi: 10.1007/s42535-019-00036-1
Doi Link: https://doi.org/10.1007/s42535-019-00036-1
First Page: 345
Last Page: 352
Published: 29 June, 2019

Biochemical alterations in Aloe vera (L.) Burm. f. leaves infected with Colletotrichum gloeosporioides


Abstract:

This study aimed to determine the alterations in major biochemical contents of Aloe vera infested by Colletotrichum gloeosporioides. Effects of fungal pathogen C. gloeosporioides on chlorophyll-a, chlorophyll-b, total chlorophyll, total soluble sugar, total phenols, total anthraquinones, total flavonoids, carotenoids and vitamin E were analyzed in artificially infested leaves of A. vera. While significant decrease in the contents of chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoids and total soluble sugar was observed, an increased concentration of total phenolics, total anthraquinones, total flavonoid and vitamin E contents was recorded in infested leaves as compared to control. Being a medicinal plant, A. vera suffers a huge loss in its yield due to the attack of number of fungal pathogens. Moreover, changes in the concentration of its main biochemical groups were directly associated with the adverse effect of pathogens during the progression of infection on plant leaves. Therefore, control of the fungal diseases by applying appropriate management strategies is the important thrust to protect the plant of an enormous commercial value.

Vegetos

Keywords:


n Aloe veran , n Colletotrichum gloeosporioidesn , Leaf spot, Biochemical groups, Phenolics, Chlorophyll, Vitamin E


References:


  1. Alberto RT (2014) Pathological response and biochemical changes in Allium cepa L. (bulb onions) infected with anthracnose-twister disease. Plant Pathol Quar 4(1):23–31

  2. Alkahtani M, El- Naggar MA, Omer SA, Abedl- Kareem EM, Ammar MI (2011) Effect of toxic Fusarium moniliforme on some biochemical component of some date palm cultivars. Am J Food Technol 6(9):730–741

  3. Arnon DI (1949) Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiol 2:1–15

  4. Avasthi S, Gautam AK, Bhadauria R (2018) Changes in biochemical components in Aloe vera (L.) Burm. f. leaves infected with Fusarium proliferatum (Matsushima) Nirenberg. J Appl Res Med Aromat Plant 11:61–66

  5. Bajwa R, Mukhtar I, Mushtaq S (2010) New report of Alternaria alternata causing leaf spot of Aloe vera in Pakistan. Can J Plant Pathol 32(4):490–492

  6. Bieri JG, Teets L, Belavady B, Andrews EL (1964) Serum vitamin E levels in a normal adult population in the Washington, DC. area. Proc Soc Exp Biol Med 117:131–133

  7. Boudreau MD, Beland FA (2006) An evaluation of the biological and toxicological properties of Aloe Barbadensis (Miller), Aloe vera. J Environ Sci Health 24:103–154

  8. Campos M, Cordi L, Duran N, Mei L (2006) Antibacterial activity of chitosan solutions for wound dressing. Macromol Symp 1(5):15–18

  9. Chen S, Dai X, Qiang S, Tang Y (2005) Effect of a nonhost-selective toxin from Alternaria alternata on chloroplast-electron transfer activity in Eupatorium adenophorum. Plant Pathol 54:671–677

  10. Ciuffetti LM, Manning VA, Pandelova I, Betts MF, Martinez JP (2010) Host-selective toxins, Ptr ToxA and Ptr ToxB, as necrotrophic effectors in the Pyrenophora triticirepentis-wheat interaction. New Phytol 187:911–919

  11. Dai XB, Chen SG, Qiang S, An QF, Zhang RX (2004) Effect of toxin from Alternaria alternata (Fr.) Keissler on leaf photosynthesis of Eupatorium adenophorum Spreng. Acta Phytopathol Sin 34:55–60

  12. Das S (2011) Isolation and characterization of novel protein with anti-fungal and antiinflammatory properties from Aloe vera leaf gel. Int J Biol Macromol 48(1):38–43

  13. Dewanto V, Wu X, Adom KK, Liu RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 50:3010–3014

  14. Dubey R, Pandey AK (2009) A new report on collar and root rot disease of Aloe vera caused by Sclerotium rolfsii. J Mycol Plant Pathol 39:300–301

  15. Dubios MK, Gilles JK, Robers PA, Smith F (1951) Colorimetric determination of sugar and related substance. Anal Chem 26:351–356

  16. Esser RP, Riherd CC, Harkcom KJ (1986) Research notes—pathogenicity of Scutellonema brachyurum to Aloe vera. Nematropica 16:65–71

  17. Farkas GL, Kiraly Z (1962) Role of phenolic compounds in the physiology of plant diseases and disease resistance. J Phytopathol 44:105–150

  18. Flora G, Rani SMV (2013) Effect of Stoechospermum marginatum (Ag). Kutz extract on controlling blast disease in rice caused by Pyricularia oryzae under natural field condition. Afr J Agric Res 8:3289–3293

  19. Ghosh L, Neela FA, Mahal MF, Khatun MJ, Ali MR (2012) Effect of various factors on the development of leaf spot disease in Mulberry. J Environ Sci Nat Res 5(1):205–209

  20. Gupta S, Masood A (2003) Leaf spot disease of Indian Aloe (Aloe vera Linn. Syn. Aloe barbadensis Mill.) caused by Alternaria alternata. In: Proceedings of national symposium on emerging trends in Indian medicinal plants, Lucknow, 10–12 October 2003

  21. Hamman JH (2008) Composition and applications of Aloe vera leaf gel. Molecules 13:1599–1616

  22. Haque MZ, Jalil MA, Islam MB (2012) Phyto-chemical and anti-bacterial screening of musabbar prepared from Aloe vera. J Adv Sci Res 3(4):74–77

  23. Hossain MTO, Alam MZ, Absar N (1999) Changes in different nutrients and enzyme contents in mango leaves infected with Colletotrichum gloeosporioides. Ind Phytopathol 52(1):75–76

  24. Jin KS, Lee SW, Kim JJ, Ryu HY (1994) Identification of bacterial isolates obtained from diseased orchid and Aloe plants caused by Erwinia chrysanthemi. RDA J Agric Sci Crop Prot 36:301–306

  25. Khatri RK, Shastry RP, Reddy PN, Nema KG (1985) Metabolic changes in rice leaves infected by Entyloma oryzae. Indian Phytopathol 38(4):769–771

  26. Li Z, Zhong H, Peng X, Li J, Zheng J (2008) Effect of chitosan and CaCl2 on senescence and membrane lipid peroxidation of postharvest kumquat fruits. Acta Hortic 769:259–264

  27. Lobato AKS, Gonçalves-Vidigal MC, Filho VPS, Costa RCL, Cruz FJR, Santos DGC, Silva CR, Silva LI, Sousa LL (2009) Changes in photosynthetic pigment and carbohydrate content in common bean cultivars infected by Colletotrichum lindemuthianum. Plant Soil Environ 55(2):58–61

  28. Mahadevan A, Sridhar R (1982) Methods in physiological plant pathology, 2nd edn. Sivakami Publication, Perungudi, pp 157–159

  29. Maia FGM, Ogoshi C, Vieira JF, Pierre RO, Maia JB, Junior PMR, De Abreu MS (2012) Pigments, total soluble phenols and lignin levels of coffee seedlings inoculated with Colletotrichum gloeosporioides. Coffee Sci Lavras 7(2):152–159

  30. Majumdar VL, Bhatnagar K, Sharma H, Verma OP (2007) Three new diseases of Aloe barbadensis L. J Mycol Plant Pathol 37:124–125

  31. Medhin LB, Sibhatu DB, Seid M, Ferej FM, Mohamedkassm N, Berhane Y, Kaushek A, Humida ME, Gasmalbari E (2019) Comparative antimicrobial activities of the gel, leaf and anthraquinone fractionates of four Aloe Species (Aloe camperi, Aloe elegans, Aloe eumassawana and Aloe scholleri). AD Microbiol 9:139–150

  32. Mengane SK, Kamble SS (2012) Changes in biochemical composition of soybean leaves infected with resistant and sensitive isolates of Phakopsora pachyrhizi causing soybean rust. Deccan Curr Sci 7:122–125

  33. Misra RS, Sharma K, Mishra AK, Sriram S (2008) Biochemical alterations induced in taro in response to Phytophthora colocasiae infection. Adv Nat Appl Sci 2(3):112–121

  34. Naik MK, Hiremath PC, Hiremath SV (1988) The metabolic changes in the plant tissue infected by fungal attack. Indian Phytopathol 41:370–372

  35. Neish AC (1964) Major pathways of biosynthesis of phenols. In: Harbone JB (ed) Biochemistry of phenolic compounds. Academic Press, New York, pp 295–359

  36. Nema AG (1989) Sugar and phenol contents of betelvine leaves after inoculation with leaf spot bacterium. Indian Phytopathol 42(1):31–37

  37. Noveroske RL, Williams EB, Kuc J (1964) 7-Glucosidase and polyphenol oxidise in apple leaves and their possible relation Venturia inaequallis. J Phytopathol 54:98–103

  38. Pandey M, Trivedi S, Gurha SN, Tripathi VK (2008) Dry rot and leaf spot of Aloe barbadensis caused by Alternaria alternata. Ann Plant Prot Sci 16(2):512–513

  39. Parashurama TR, Mahishi P, Mallikarjunaswamy GE, Byrappa SM (2013) Cercospora leaf spot disease in Centella asiatica and its effect on pharmaceutical components. Int J Biol Pharm Res 4(1):64–71

  40. Petkovsek MM, Schmitzer V, Jakopic J, Cunja V, Veberic R, Munda A, Stampar F (2014) Colletotrichum lindemuthianum infection causes changes in phenolic content of French green bean pods. Sci Hortic 170:211–218

  41. Prasad B, Verma OP, Daftari LN (1976) Biochemical changes in safflower leaves caused by rust infection. Indian Phytopathol 29(1):53–58

  42. Pritam A, Kale PG (2007) Alteration in the antioxidant potential of Aloe vera due to fungal infection. J Plant Pathol 6(2):169–173

  43. Ravi S, Kabilar P, Velmurugan S, Kumar RA, Gayathiri M (2011) Spectroscopy studies on the status of aloin in Aloe vera and commercial samples. J Exp Sci 2(8):10–13

  44. Reddy BC, Reddy PV, Gopala RP (1984) Post infection changes in acid lime fruits caused Aspergillus niger. Indian Phytopathol 37(1):185–187

  45. Ritchie HE (2001) The safety of herbal medicine use during pregnancy. Front Fetal Health 3(10):259–266

  46. Sakulpanich A, Gritsanapan W (2008) Extraction method for high content of anthraquinones from Cassia fistula pods. J Health Res 22(4):167–172

  47. Shivanna MB, Mallikarjunaswamy GE (2009) Fungal diseases and their effect on phytochemical constituents of medicinally important Terminalia species in Bhadra Wildlife Sanctuary, Karnataka, India. Indian Phytopathol 62(1):37–43

  48. Swarbrick PJ, Schulze-Lefert P, Scholes JD (2006) Metabolic consequences of susceptibility and resistance in barley leaves challenged with powdery mildew. Plant Cell Environ 29:1061–1076

  49. Tamuli P, Boruah P, Samanta R (2004) Biochemical changes in essential oil bearing Aquilaria malaccensis lamk. under pathogenesis. J Spices Aromat Crops 13(2):87–91

  50. Tamuli P, Boruah P, Samanta R (2013) Post-infectional biochemical changes in Cymbopogon martinii (Roxb.) Wats and Cymbopogon citratus (DC) Stapf. due to leaf rust disease. Am J Plant Sci 4:1666–1668

  51. Verma OP, Singh N, Sharma P (2006) Dry rot of leaf—a new symptom caused by Alternaria alternara on Indian Aloe. J Mycol Plant Pathol 36:302–303

  52. Vidyasagar GM, Kotresha D (2003) Biochemical changes due to infection of Phyllactinia corylea in the leaves of certain Mulberry varieties. Indian J Seric 42:71–74

  53. Yang TC, Chou CC, Li CF (2005) Antibacterial activity of N-alkylated disaccharide chitosan derivatives. Int J Food Microbiol 97(1):237–245

  54. Zhao J, Zhao F, Jian G, Ye Y, Zhang W, Li J, Qi F (2013) Intensified Alternaria spot disease under potassium deficiency conditions results in acceleration of cotton (Gossypium hirsutum L.) leaf senescence. Aus J Crop Sci 7:241–248

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Acknowledgements :



Authors express their sincere thanks to Head, School of Studies in Botany, Jiwaji University Gwalior, Madhya Pradesh, India for providing essential laboratory facilities and support to conduct this research work successfully.


Author Information:



Shubhi Avasthi
School of Studies in Botany, Jiwaji University, Gwalior, India
shubh.avasth@gmail.com
Ajay Kumar Gautam
School of Agriculture, Faculty of Science, Abhilashi University, Mandi, India


Rekha Bhaduaria
School of Studies in Botany, Jiwaji University, Gwalior, India




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