VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 3, September 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 265

Doi: 10.1007/s42535-019-00048-x
Doi Link: https://doi.org/10.1007/s42535-019-00048-x
First Page: 387
Last Page: 396
Published: 30 July, 2019

Altitudinal variation in gallic acid content in fruits of Phyllanthus emblica L. and its correlation with antioxidant and antimicrobial activity


Abstract:

Gallic acid is one of the most important organic compounds in fruits of Phyllanthus emblica. Therefore, the objective of the present study is to find out the effect of altitude on gallic acid content in fruits of P. emblica and its correlation with antioxidant and antimicrobial activity. Phytochemicals such as phenolics, tannins, flavonoids, carbohydrates, glycosides, phytosteroids, alkaloids and saponins were detected in fruit extracts of P. emblica collected from different regions of Himachal Pradesh. Fruits extract from Mandi (239.74 ± 39.28 mg/g gallic acid equivalents, GAE) district showed a higher amount of total phenolic content (TPC), whereas, total flavonoid content (TFC) was higher from Kangra (356 ± 27.63 mg/g rutin equivalents, RE) district. Methanolic extracts showed inhibition to the growth of both Gram-positive (B. subtilis, S. aureus), and Gram-negative bacteria (E. coli, K. pneumoniae). The methanolic extract of fruits of Bilaspur district showed the highest antibacterial activity against B. subtilis (19.5 ± 0.71 mm), S. aureus (21.0 ± 1.41 mm), E. coli (17.5 ± 0.71) and K. pneumoniae (21.5 ± 2.12) as compared to other regions and amoxyclav. High-performance thin-layer chromatography (HPTLC) method was used for the quantification of gallic acid in the extracts of fruits of P. emblica. HPTLC chromatogram showed the highest content of gallic acid in methanolic extracts of fruits from Kangra followed by Bilaspur, Mandi, and Una. However, antioxidant and antibacterial activity was higher in fruits extracts of high altitude (Bilaspur region). In summary, Bilaspur region of Himachal Pradesh could be used for mass cultivation of fruits of P. emblica because of their high antioxidant and antimicrobial potential under these geographical conditions.

Vegetos

Keywords:


Antibacterial, Antioxidant, HPTLC, n Phyllamthus emblican , Phytochemical variation


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Acknowledgements :



The authors acknowledge Shoolini University, Solan, for providing infrastructure support to conduct the research work. Authors also acknowledge the support provided by Yeast Biology Laboratory, School of Biotechnology and Central Instrumentation laboratory, School of Pharmaceutical Sciences, Shoolini University, Solan, Himachal Pradesh, India.


Author Information:



Shagun Sheoran
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India

Prakriti Nidhi
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India


Vikas Kumar
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India

Gajender Singh
School of Pharmaceutical Sciences, Shoolini University of Biotechnology and Management Sciences, Bajhol, India

Uma Ranjan Lal
Department of Natural Products, National Institute of Pharmaceutical Education, Ahmedabad, India

Anuradha Sourirajan
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India
asourirajan@gmail.com
Kamal Dev
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, India
kamaldevbhardwaj1969@gmail.com




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