VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 3, September 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 35

Doi: 10.1007/s42535-019-00051-2
Doi Link: https://doi.org/10.1007/s42535-019-00051-2
First Page: 431
Last Page: 438
Published: 02 August, 2019

Morphological and biochemical responses of Phaseolus vulgaris L. to mineral stress under in vitro conditions


Abstract:

Mineral stress is one of the major abiotic stresses faced by crop plants. The present study was conducted to investigate the impact of mineral stress (Fe and Zn) on various morphological and biochemical responses of the shoot and root tissues of common bean (Phaseolus vulgaris L.). The study was conducted under in vitro conditions, in which seeds of SFB-1 (Shalimar French Bean-1) were cultured on four different MGRL medium (control, 0-Fe, 0-Zn and 300-Zn). Chlorophyll, total sugars, proline, length and weight of shoot and root were assessed and compared within and between the treatments. The data analyzed revealed significant difference between control and other three treatments. Length and weight of shoot and root significantly decreased under 0-Fe, 0-Zn and 300-Zn as compared to control. Chlorophyll content was significantly decreased in three treatments 0-Fe, 0-Zn and 300-Zn than control. Total sugar was significantly higher in shoots and 300-Zn of roots in comparison to control. Proline content was significantly higher in both tissues for 0-Fe, 0-Zn and 300-Zn than control. These findings suggest disturbance in cell homeostasis leading to damage/ oxidative stress in response to mineral imbalances, however, further investigations are required to understand changes in gene expression levels under Fe and Zn stress.

Vegetos

Keywords:


Mineral stress, Common bean, Biochemical, Morphology, n Phaseolus vulgarisn , In vitro


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Acknowledgements :



We are grateful to Dean, Faculty of Horticulture, SKUAST-Kashmir for support.


Author Information:



Uneeb Urwat
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Sajad Majeed Zargar
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India
smzargar@gmail.com

Madhiya Manzoor
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Syed Mudasir Ahmad
Division of Animal Biotechnology, FVSc, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Nazir Ahmad Ganai
Division of Animal Biotechnology, FVSc, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Imtiyaz Murtaza
Division of Basic Sciences and Humanities, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Imran Khan
Division of Agri Statistics, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

, F. A. Nehvi
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India




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