VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles


Volume: 32, Issue: 3, September 2019

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Page Visits: 67

Doi: 10.1007/s42535-019-00051-2
Doi Link:
First Page: 431
Last Page: 438
Published: 02 August, 2019

Morphological and biochemical responses of Phaseolus vulgaris L. to mineral stress under in vitro conditions


Mineral stress is one of the major abiotic stresses faced by crop plants. The present study was conducted to investigate the impact of mineral stress (Fe and Zn) on various morphological and biochemical responses of the shoot and root tissues of common bean (Phaseolus vulgaris L.). The study was conducted under in vitro conditions, in which seeds of SFB-1 (Shalimar French Bean-1) were cultured on four different MGRL medium (control, 0-Fe, 0-Zn and 300-Zn). Chlorophyll, total sugars, proline, length and weight of shoot and root were assessed and compared within and between the treatments. The data analyzed revealed significant difference between control and other three treatments. Length and weight of shoot and root significantly decreased under 0-Fe, 0-Zn and 300-Zn as compared to control. Chlorophyll content was significantly decreased in three treatments 0-Fe, 0-Zn and 300-Zn than control. Total sugar was significantly higher in shoots and 300-Zn of roots in comparison to control. Proline content was significantly higher in both tissues for 0-Fe, 0-Zn and 300-Zn than control. These findings suggest disturbance in cell homeostasis leading to damage/ oxidative stress in response to mineral imbalances, however, further investigations are required to understand changes in gene expression levels under Fe and Zn stress.



Mineral stress, Common bean, Biochemical, Morphology, n Phaseolus vulgarisn , In vitro


  1. Ahanger MA, Akram NA, Ashraf M, Nasser M, Alyemeni AL, Ahmad P (2017) Plant responses to environmental stresses—from gene to biotechnology. AoBP 9(4):plx025.


  3. Amirjani MR (2011) Effect of salinity stress on growth, sugar content, pigments and enzyme activity of rice. Int J Bot 7(1):73–81.

  4. Aono M, Kubo A, Saji H, Tanaka K, Kondo N (1993) Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity. Plant Cell Physiol 34(1):129–135.

  5. Baldrich CA, Bosch N, Begines D, Feria AB, Monreal JA, Maurino SG (2015) Proline synthesis in barley under iron deficiency and salinity. J Plant Physiol. 183:121–129.

  6. Bashir K, Rasheed S, Kobayashi T, Seki M, Nishizawa NK (2016) Regulating subcellular metal homeostasis: The key to crop improvement. Front Plant Sci 7:1192.

  7. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

  8. Bavaresco L, Fregoni M, Perino A (1994) Physiological aspects of lime-induced chlorosis in some Vitis species. I. Pot trial on calcareous soil. Vitis 33:123–126

  9. Bocchini M, Bartucca ML, Ciancaleoni S, Mimmo T, Cesco S, Pii Y, Albertini E, Del Buono D (2015) Iron deficiency in barley plants: phytosiderophore release, iron translocation, and DNA methylation. Front Plant Sci. 6:514.

  10. Brown PH, Cakmak I, Zhang Q (1993) Form and function of zinc in plants. In: Robson AD (ed) Zinc in soils and plants. Kluwer Academic Publishers, Dordrecht, pp 93–106

  11. Chen XY, He YF, Luo YM, Yu YL, Lõn Q, Wong MH (2003) Physiological mechanism of plant roots exposed to cadmium. Chemosphere 50:789–793.

  12. Guerrero NAC, Arellano MCI, Cozatl DGM, López VO (2016) Common bean: a legume model on the rise for unraveling responses and adaptations to iron, zinc, and phosphate deficiencies. Front Plant Sci 7:600.

  13. Hajiboland R, Amirazad F (2010) Growth, photosynthesis and antioxidant defense system in Zn-deficient red cabbage plants. Plant Soil Environ 56(5):209–217.

  14. Hasaneen MNA, Younis ME, Tourky SMN (2009) Plant growth, metabolism and adaptation in relation to stress conditions XXIII. Salinity-biofertility interactive effects on growth, carbohydrates and photosynthetic efficiency of Lactuca sativa. Plant Omics 2:60–69

  15. Houmani H, Jelali N, Abdelly C, Gharsalli M (2012) Mineral elements bioavailability in the halophyte species Suaedafruticosa. J Biol Res-Thessalon 17:113–120

  16. Husen A, Iqbal M, Aref IM (2016) IAA induced alteration in growth and photosynthesis of pea (L.) plants grown under salt stress Pisum sativum. J Environ Biol 37:421–429

  17. Inskeep WP, Bloom PR (1985) Extinction coefficients of chlorophyll a and B in n, n-dimethylformamide and 80% acetone. Plant Physiol 77(2):483–485.

  18. Jelali N, Dell’Orto M, Rabhi M, Zocchi G, Abdelly C, Gharsalli M (2010) Physiological and biochemical responses for two cultivars of Pisumsativum (‘‘Merveille de Kelvedon’’ and‘‘Lincoln’’) to iron deficiency conditions. Sci Hort 124:116–121.

  19. Jin ZM, Wang CH, Liu ZP, Gong WJ (2007) Physiological and ecological characters studies on Aloe vera under soil salinity and seawater irrigation. Process Biochem 42(4):710–714.

  20. Khateeb W, Qwasemeh H (2014) Cadmium, copper and zinc toxicity effects on growth, proline content and genetic stability of Solanum nigrum L., a crop wild relative for tomato; comparative study. Physiol Mol Biol Plants 20(1):31–39.


  22. Lin XY, Ye YQ, Fan SK, Jin CW, Zheng SJ (2016) Increased Sucrose accumulation regulates iron-deficiency responses by promoting Auxin signaling in Arabidopsis plants. Plant Physiol 170:907–920.

  23. Lobato AKDS, Lima EJA, Lobato EMSG, Maciel GM, Marques DJ (2016) Tolerance of plants to toxicity induced by micronutrients, abiotic and biotic stress in plants. In: Shanker AK, Shanker C (eds) Recent advances and future perspectives. IntechOpen.

  24. Mahmoudi H, Labidi N, Ksouri R, Gharsalli M, Abdelly C (2007) Differential tolerance to iron deficiency of chickpea varieties and Fe resupply effects. CR Biol 330:237–246.

  25. Mandre M, Tullus H, Kloseiko J (2002) Partitioning of carbohydrates and biomass of needles in Scots pine canopy. Z Naturforsch C 57(3–4):296–302

  26. Molassiotis A, Tanou G, Diamantidis G, Patakas A (2006) The rios Effects of 4-month Fe deficiency exposure on Fe reduction mechanism, photosynthetic gas exchange, chlorophyll fluorescence and antioxidant defense in two peach rootstocks differing in Fe deficiency tolerance. J Plant Physiol 163:176–185.

  27. Mousavi SR (2011) Zinc in crop production and interaction with phosphorus. Aust J Basic Appl Sci 5(9):1503–1509

  28. Paula LS, Silva BC, Pinho WCS, Barbosa MAM, Lobato EMSG, Batista BL, Barbosa JF (2015) Lobato AKS (2015) Silicon (Si) ameliorates the gas exchange andreduces negative impacts on photosynthetic pigments in maize plants under Zn toxicity. Aust J Crop Sci 9(10):901–908

  29. Pessarakli M, Haghighi M, Sheibanirad A (2015) Plant responses under environmental stress conditions. Adv Plants Agric Res 2(6):00073.

  30. Radić S, Babić M, Skobič D, Rojec V, Pevalek KB (2009) Ecotoxicological effects of aluminum and zinc on growth and antioxidants in Lemna minor L. Ecotoxicol Environ Saf 73(3):336–342.

  31. Sami F, Yusuf M, Faizan M, Faraz A, Hayat S (2016) Role of sugars under abiotic stress. Plant Physiol Biochem 109:54–61.

  32. Sandhya V, Ali SKZ, Grover M, Reddy G, Venkateswarlu B (2010) Effect of plant growth promoting Pseudomonas spp on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regul 62(1):21–30

  33. Sbai H, Haouala R (2018) Responses of two Apiaceae species to direct iron deficiency. J Photochem Photobiol 2(1):16–21.


  35. Sharma P, Dubey RS (2005) Lead toxicity in plants. Braz J Plant Physiol 17(1):35–52.

  36. Shri PU (2017) Proline accumulation under Zinc stress in Sorghum bicolor (L) Plants. IJSAR 4(9):01–06

  37. Sibley JL, Eakes DJ, Gilliam CH, Keever GJ, Dozier WA, Himelrick DG (1996) Foliar SPAD-502 meter values, nitrogen levels, and extractable chlorophyll for red maple selections. Hort Sci 31(3):468–470.

  38. Sirousmehr A, Arbabi J, Asgharipour MR (2014) Effect of drought stress levels and organic manures on yield, essential oil content and some morphological characteristics of sweet basil (Ocimum basilicum). Adv Environ Biol 8(4):880–886

  39. Subba P, Mukhopadhyay M, Mahato SK, Bhutia KD, Mondal TK, Ghosh SK (2014) Zinc stress induces physiological, ultra-structural and biochemical changes in mandarin orange (Citrus reticulata Blanco) seedlings. Physiol Mol Biol Plants 20(4):461–473.

  40. Vassilev A, Nikolova A, Koleva L, Lidon F (2011) Effects of Excess Zn on growth and photosynthetic performance of young Bean plants. J. phytol 3(6):58–62

  41. Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Golezani KG (2011) Physiological responses of soybean (Glycine max L.) to zinc application under salinity stress. Aust J Crop Sci 11:1441–1447

  42. Zargar SM, Agrawal GK, Rakwal R, Fukao Y (2015) Quantitative proteomics reveals role of sugar in decreasing photosynthetic activity due to Fe deficiency. Front Plant Sci 6:592.

  43. Zargar SM, Mahajan R, Nazir M, Nagar P, Kim ST, Rai V, Masi A, Ahmad SM, Shah RA, Ganai NA, Agrawal GK, Rakwal R (2017) Common bean proteomics: present status and future strategies. J Proteom 169:239–248.


Acknowledgements :

We are grateful to Dean, Faculty of Horticulture, SKUAST-Kashmir for support.

Author Information:

Uneeb Urwat
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Sajad Majeed Zargar
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Madhiya Manzoor
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Syed Mudasir Ahmad
Division of Animal Biotechnology, FVSc, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Nazir Ahmad Ganai
Division of Animal Biotechnology, FVSc, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Imtiyaz Murtaza
Division of Basic Sciences and Humanities, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

Imran Khan
Division of Agri Statistics, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

, F. A. Nehvi
Division of Plant Biotechnology, SK University of Agricultural Sciences and Technology of Kashmir, Srinagar, India

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