VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 32, Issue: 1, March 2019


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 34

Doi: 10.1007/s42535-019-00006-7
Doi Link: https://doi.org/10.1007/s42535-019-00006-7
First Page: 48
Last Page: 57
Published: 22 February, 2019

Analysis of genetic variation using ISSR and the development of SCAR marker in synthetic autotetraploids of Vigna mungo (L.) Hepper


Abstract:

Autopolyploidy is one of the foremost mechanisms by which additional numbers of genome copies are acquired resulting in genetic and evolutionary novelty to the organisms. As such, it is considered to be a pathway for adaptation and has played a significant role in plant speciation. Autopolyploids are characterized by increased genome flexibility, allowing them to adapt and persist across heterogeneous landscapes in the long run. Genomic redundancy and polysomic inheritance are the hallmarks of doubled genome(s). In view of the significance of autopolyploidy in plant evolution, plant breeding and crop improvement, we sought to investigate the genetic variation/s associated with genome multiplication by exploiting artificial somatic autopolyploids in Vigna mungo. ISSR was employed to detect and identify any genomic DNA variation in colchitetraploids of V. mungo of three different generations, viz. C0, C1 and C2, in comparison to their putative diploids plants. Our data suggests a random, ‘short-term’ rapid change of the genome that may have occurred immediately after polyploidization. The population of autopolyploids demonstrated some degree of genetic heterozygosity in comparison to their diploid counterparts. Further, a polymorphic ISSR DNA loci was identified among the colchitetraploids which was translated into SCAR marker for identification of colchitetraploids versus the putative diploids of V. mungo.

Vegetos

Keywords:


Autopolyploidy, Genetic variation, DNA marker, ISSR, SCAR


References:


  1. Barker MS, Arrigo N, Baniaga AE, Li Z, Levin DA (2016) On the relative abundance of autopolyploids and allopolyploids. New Phytol 210:391–398

  2. Chen ZJ (2007) Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids. Annu Rev Plant Biol 58:377–406

  3. Chen ZJ, Ni Z (2006) Mechanisms of genomic rearrangements and gene expression changes in plant polyploids. BioEssays 28:240–252

  4. Comai L (2005) The advantages and disadvantages of being polyploid. Nat Rev Genet 6:836–846

  5. Cronn RC, Small RL, Wendel JF (1999) Duplicated genes evolve independently after polyploid formation in cotton. Proc Natl Acad Sci USA 96:14406–14411

  6. Cunha CM, Hinz RH, Pereira A, Tcacenco FA, Paulino EC, Stadnik MJ (2015) A SCAR marker for identifying susceptibility to Fusariumoxysporum f. sp. cubense in banana. Sci Hortic 191:108–112

  7. Dar TH, Raina SN, Goel S (2013) Molecular analysis of genomic changes in synthetic autotetraploidPhlox drummondii Hook. Biol J Linnean Soc 110:591–605

  8. Dar TH, Raina SN, Goel S (2017) Cytogenetic and molecular evidences revealing genomic changes after autopolyploidization: a case study of synthetic autotetraploidPhlox drummondii Hook. Physiol Mol Biol Pla 23:641–650

  9. Das K, Ganie SH, Mangla Y, Chaudhary M, Thakur RK, Tandon R, Raina SN, Goel S (2017) ISSR markers for gender identification and genetic diagnosis of Hippophaerhamnoides ssp. turkestanica growing at high altitudes in Ladakh region (Jammu and Kashmir). Protoplasma 254:1063–1077

  10. Gao R, Wang H, Dong B, Yang X, Chen S, Jiang J, Zhang Z, Liu C, Zhao N, Chen F (2016) Morphological, genome and gene expression changes in newly induced autopolyploid Chrysanthemum lavandulifolium (fisch. Ex trautv.) makino. Int J Mol Sci 17:1690–1702

  11. Hegarty MJ, Hiscock SJ (2008) Genomic clues to the evolutionary success of polyploid plants. Curr Biol 18:435–444

  12. Jaccard P (1901) Étude comparative de la distribution floraledansune portion des Alpeset des Jura. Bull Soc Vaud Sci Nat 37:547–579

  13. Leitch IJ, Bennett MD (2004) Genome downsizing in polyploid plants. Biol J Linn Soc 82:651–663

  14. Liu S, Yang Y, Wei F, Duan J, Braynen J, Tian B, Cao G, Shi G, Yuan J (2017) Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana. Theor Biosci 136:199–206

  15. Mable BK, Beland J, Di Berardo C (2004) Inheritance and dominance of self-incompatibility alleles in polyploid Arabidopsis lyrata. Heredity 93:476–486

  16. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326

  17. Otto SP (2007) The evolutionary consequences of polyploidy. Cell 131:452–462

  18. Parisod C, Holderegger R, Brochmann C (2010) Evolutionary consequences of autopolyploidy. New Phytol 186:5–17

  19. Del Pozo JC, Ramirez-Parra E (2015) Whole genome duplications in plants: an overview from Arabidopsis. J Exp Bot 66:6991–7003

  20. Rajesh MK, Jerard BA, Preethi P, Thomas RJ, Fayas TP, Rachana KE, Karun A (2013) Development of a RAPD-derived SCAR marker associated with tall-type palm trait in coconut. Scientia Hortic 150:312–316

  21. Rao SR, Raina SN (2005) Cytological evalutation of colchitetraploidy in moth bean (Vigna aconitifolia) and its allied species. J Arid Legume 2:389–396

  22. Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

  23. Rieseberg LH (2001) Polyploid evolution: keeping the peace at genomic reunions. Curr Biol 11:R925–928

  24. Rohlf FJ (1998) NTSYSpc. Version 2.10p. Applied biostatistics, Setauket, NY 11733–2870, USA. Available from: https://www.exetersoftware.com/cat/ntsyspc/ntsyspc.html

  25. Sairkar PK, Sharma A, Shukla NP (2016) SCAR marker for identification and discrimination of Commiphora wightii and C. myrrha. Mol Biol Int 1:2. https://doi.org/10.1155/2016/1482796

  26. Sattler MC, Carvalho CR, Clarindo WR (2016) The polyploidy and its key role in plant breeding. Planta 243:281–296

  27. Soltis DE, Soltis PS (1999) Polyploidy: recurrent formation and genome evolution. Trends Ecol Evol 14:348–352

  28. Soltis PS, Soltis DE (2000) The role of genetic and genomic attributes in the success of polyploids. Proc Natl Acad Sci USA 97:7051–7057

  29. Soltis DE, Soltis PS, Rieseberg LH (1993) Molecular data and the dynamic nature of polyploidy. Crit Rev Plant Sci 12:243–273

  30. Spoelhof JP, Soltis PS, Soltis DE (2017) Pure polyploidy: closing the gaps in autopolyploid research. J Syst Evol 55:340–352

  31. Stupar RM, Bhaskar PB, Yandell BS, Rensink WA, Hart AL, Ouyang S, Veilleux RE, Busse JS, Erhardt RJ, Buell CR, Jiang J (2007) Phenotypic and transcriptomic changes associated with potato autopolyploidization. Genetics 176:2055–2067

  32. Wang J, Tian L, Lee HS, Wei NE, Jiang H, Watson B, Madlung A, Osborn TC, Doerge RW, Comai L, Chen ZJ (2006) Genomewidenonadditive gene regulation in Arabidopsis allotetraploids. Genetics 172:507–517

  33. Wu M, Zhang JP, Wang JC, Yang XM, Gao AN, Zhang XK, Liu WH, Li LH (2010) Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of Agropyron cristatum. Euphytica 172:363–372

  34. Yu Z, Haage K, Strei VE, Gierl A, Torres Ruiz RA (2009) A large number of tetraploid Arabidopsis thaliana lines, generated by a rapid strategy, reveal high stability of neo-tetraploids during consecutive generations. Theor Appl Genet 118:1107–1119

  35. Yuskianti V, Shiraishi S (2010) Sequence characterized amplified region (SCAR) markers in Sengon (Paraseriathes falcataria (L.) Nielsen. HAYATI J Biosci 17:167–172

  36. Zhang X, Ma HX, Zhou YJ, Xing JC, Chen JH, Yu GH, Sun XB, Wang L (2014) Identification and genetic division of Fusarium graminearum and Fusarium asiaticum by species-specific SCAR markers. J Phytopathol 162:81–88


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Acknowledgements :



The present work was carried out in Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong. The authors are thankful to Indian Institute of Pulses Research, Kanpur for providing the germplasm, Department of Biotechnology, Government of India and Ministry of Tribal Affairs, Government of India– National fellowship for Higher education of ST students’ scheme for financial assistance and fellowship grants. Sincere appreciations are also due to the members of Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong for their constant help and encouragement.


Author Information:



Daniel Regie Wahlang
Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India

Wanfulmi Suchiang
Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India


Shailendra Goel
Department of Botany, University of Delhi, Delhi, India

Satyawada Rama Rao
Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, India

srrao22@yahoo.com




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