VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 34, Issue: 1, March 2021


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 110

Doi: 10.1007/s42535-021-00192-3
Doi Link: https://doi.org/10.1007/s42535-021-00192-3
First Page: 19
Last Page: 28
Published: 02 February, 2021

Leaf development and anatomy of in vitro-grown Polygala paniculata L. are affected by light quality, gelling agents, and sucrose


Abstract:

Plant in vitro growth performance is influenced by environmental, physical, and chemical conditions. The present study evaluated the mutual effect of light, gelling agents, and sucrose concentration on leaf anatomical structure of in vitro-grown Polygala paniculata. Nodal segments were inoculated on agar- or Phytagel®-gelified medium supplemented with 15 g L−1 or 30 g L−1 sucrose. Cultures were grown under fluorescent lamps, light-emitting diodes (LEDs) with white light, and LEDs with blue and red light at 56 µmol m−2 s−1 photon flux. After 45 days of culture, leaf histology was assessed. The leaves of P. paniculata had an uniseriate epidermis and dorsiventral mesophyll. Gelling agents had antagonistic effects in relation to sucrose concentration. Growth on agar and 30 g L−1 sucrose induced greater leaf blade thickness, with expanded palisade and spongy parenchyma but looser intercellular spaces. In contrast, growth on Phytagel® led to significantly thicker palisade parenchyma at 15 g L−1 sucrose. Fluorescent lamp light favored in vitro leaf development of P. paniculata grown on agar; whereas spectral quality had no effect on leaf blade thickness in Phytagel®-grown plants. In summary, mutual combinations of gelling agent, light quality, and sucrose concentration influence the establishment and anatomic structure of leaves in P. paniculata plantlets grown in vitro. This result will facilitate studies on in vitro propagation of this important medicinal species.

Vegetos

Keywords:


Agar, Leaf anatomy, Light quality, Micropropagation, Phytagel®n


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  61.  


Acknowledgements :



We thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (420913/2018-1).


Author Information:



Lays Araújo Nery
Instituto Federal do Norte de Minas Gerais-IFNMG, Almenara, Brazil

Diego Silva Batista
Departamento de Agricultura, Universidade Federal da Paraíba, Bananeiras, Brazil


Diego Ismael Rocha
Instituto de Ciências Biológicas, Universidade Federal de Jataí, Jataí, Brazil

Sérgio Heitor Sousa Felipe
Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, Viçosa, Brazil

Matheus da Costa Queiroz
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil

Priscila Oliveira Silva
Instituto de Educação, Agricultura e Ambiente, Universidade Federal do Amazonas, Humaitá, Brazil

Marília Contin Ventrella
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil

, Wagner Campos Otoni
Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, Viçosa, Brazil
wotoni@ufv.br
Matheus da Costa Queiroz
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, Viçosa, Brazil

Priscila Oliveira Silva
Instituto de Educação, Agricultura e Ambiente, Universidade Federal do Amazonas, Humaitá, Brazil




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