VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 34, Issue: 2, June 2021


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 99

Doi: 10.1007/s42535-021-00196-z
Doi Link: https://doi.org/10.1007/s42535-021-00196-z
First Page: 383
Last Page: 389
Published: 05 March, 2021

Effect of activated charcoal and phytohormones to improve in vitro regeneration in Vanda tessellata (Roxb.) Hook. ex G. Don


Abstract:

Vanda tessellata (Roxb.) Hook. ex G. Don. (Orchidaceae) is an epiphytic orchid species, commonly known as grey orchid, and explored enormously for its significant horticultural, medicinal, phytochemical and pharmacological properties. The present study describes an in vitro culture approach via asymbiotic seed germination assisted by phytohormones and enhanced proliferation of shoots and roots using activated charcoal for large scale production of V. tessellata. The maximum frequency of seed germination (100%) was achieved on Murashige and Skoog’s (MS) medium fortified with 1.5 mg L−1 indole-3 butyric acid (IBA). The seedlings developed from the protocorms were used for further proliferation and development of multiple shoots (24.8 ± 0.52 shoots per protocorm with 5.2 ± 0.35 cm average length) on MS medium augmented with 0.5 mg L−1 each of 6-benzylaminopurine (BAP) and indole-3 acetic acid (IAA) + 100 mg L−1 activated charcoal (AC) after 4th sub-culture. The optimal rooting of individual shoots was achieved on MS medium with 1.0 mg L−1 IBA. The well-developed plantlets were hardened using soilrite® + cocopeat + coconut husk complex in a greenhouse for 4 weeks. The plantlets were acclimatized under relatively increased temperature and low humidity for 4–5 weeks in the greenhouse with 98% survival rate. The protocol can be utilized for the development of strategies for large scale stable production of V. tessellata plantlets.

Vegetos

Keywords:


Asymbiotic seed germination, Epiphytic, Grey orchid, Shoot proliferation, n Vanda tessellatan


References:


  1. Aktar S, Nasiruddin KM, Khaldun ABM (2007) Organogenesis of Dendrobium orchid using traditional media and organic extracts. J Agric Rural Dev 5:30–35

  2. Alghamdi SA (2019) Influence of mycorrhizal fungi on seed germination and growth in terrestrial and epiphytic orchids. Saudi J Biol Sci 26:495–502

  3. Baby R, Valsala PA (2019) Micropropagation protocol for Vanda hybrid ‘Sansai Blue.’ Int J Chem Stud 7:2957–2963

  4. Baby R, Valsala PA, Doddamani MB (2019) In vitro micropropagation protocol for Vanda hybrid ‘Dr. Anek.’ Int J Curr Microbiol App Sci 8:2073–2084

  5. Basu R (2010) Role of sacred groves in biodiversity conservation in Bankura District of West Bengal. J Econ Taxon Bot 34:12–17

  6. Bhattacharjee B, Islam SMS (2014) Effects of plant growth regulators on multiple shoot induction in Vanda tessellata (Roxb.) Hook. Ex G.Don. An endangered medicinal orchid. Int J Sci Nat 5:707–712

  7. Bhattacharjee B, Islam SMS (2017) Mass propagation of PLBs derived from leaf and shoots of Vanda tessellata (Roxb.) hook. Ex G. Don an endangered medicinal orchid in Bangladesh. Bangl J Bot 46:775–782

  8. Bhattacharjee B, Islam SMS, Subramaniam S (2015) Rapid development of PLBs derived from immature seeds and mass multiplication of Vanda tessellata (Roxb.) Hook. ex G. Don—a threatened orchid species in Bangladesh. Plant Tissue Cult Biotechnol 25:181–191

  9. Centeno ML, Fernandez B, Feito I, Rodriguez A (1999) Uptake, distribution and metabolism of 1-naphthaleneacetic acid and indole-3-acetic acid during callus initiation from Actinidia deliciosa tissues. J Plant Growth Regul 18:81–88

  10. Chutipaijit S, Sutjaritvorakul T (2018) Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.). Chem Spec Bioavailab 30:1–18

  11. Hinsley A, de Boer HJ, Fay MF, Gale SW, Gardiner LM, Gunasekara RS, Kumar P, Masters S, Metusala D, Roberts DL, Veldman S, Wong S, Phelps J (2018) A review of the trade in orchids and its implication for conservation. Bot J Linn Soc 1861:435–455

  12. Jiang L, Ding P, Zheng Y (2003) Effects of additives on tissue culture and rapid propagation of Dendrobium canducum. Zhong Yao Cai 26:539–541

  13.  

  14. Khan H, Belwal T, Tariq M, Atanasov AG, Devjota HP (2019) Genus Vanda: A review on traditional uses, bioactive chemical constituents and pharmacological activities. J Ethnopharmacol 229:46–53

  15. Khuraijam JS, Sharma SC, Roy RK (2017) Orchids: potential ornamental crop in north India. Int J Hortic Crop Sci Res 7:1–8

  16. Leyva-Ovalle OR, Bello-Bello JJ, Murguia-Gonzalez J, Nunez-Pastrana R, Ramirez-Mosqueda MA (2020) Micropropagation of Guarianthe skinneri(Bateman) Dressler & W E. Higging in temporary immersion systems. 3 Biotech. https://doi.org/10.1007/s13205-019-2010-3

  17. Mirani AA, Abul-Soad AA, Markhan GS (2017) In vitro rooting of Dendrobium nobile orchid: multiple responses to auxin combinations. Not Sci Biol 9:84–88

  18. Moraes L, Faria RT, Cuquel FL (2005) Activated charcoal for in vitro propagation of Brazilian orchids. Acta Hort 683:383–390

  19. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with Tobacco tissue culture. Physiol Plant 15:473–497

  20. Novak SD, Luna LJ, Gamage RN (2014) Role of auxin in orchid development. Plant Signal Behav. https://doi.org/10.4161/psb.32169

  21. Ozel CA, Khawar KM, Mirici S, Arslan O, Sebahattin O (2006) Induction of ex vitro adventitious roots on soft wood cuttings of Centaurea tchihatcheffii Fisch & Mey using indole-3-butyric acid and α-naphthalene acetic acid. Int J Agric Biol 8(1):66–69

  22. Panwar D, Ram K, Shekhawat NS (2012) In vitro propagation of Eulophia nuda Lindl., an endangered orchid. Sci Hortic 139:46–52

  23. Prakash B, Bais RT (2016) A report on colour variation in the flowers of Vanda tessellata (Roxb.) Hook. ex. G.Don—an endangered medicinal orchid. Int Res J Env Sci 5:47–49

  24. Prizao EC, Goncalves LM, Gutierre MAM, Mangolin CA, da Silva Machado MFP (2012) Activated charcoal and graphite for the micropropagation of Cattleya bicolor Lindl. and a orchid double-hybrid ‘BLC Pastoral Innocence.’ Acta Sci 34:157–161

  25. Rahman MS, Hasan MF, Das R, Hossain MS, Rahman M (2009) In vitro micropropagation of orchid (Vanda tessellata L.) from shoot tip explants. J Bio Sci 17:139–144

  26. Shamsul Islam SM, Sayeed H, Abrar Shahriyar SK, Ferdous A, Islam A (2016) Antioxident, analgesic and cytotoxic activity of methanolic extract of Vanda Roxburghii root. Int J Pharm Sci Res 7(7):2944–2950

  27. Sharma PK, Trivedi R, Purohit S (2012) Activated charcoal improves rooting in in-vitro derived Acacia leucophloea shoots. Int J Plant Dev Biol 6:47–50

  28. Subramoniam A, Gangaprasad A, Sureshkumar PK, Radhika J, Arun BK (2013) A novel aphrodisiac compound from an orchid that activates nitric oxide synthases. Int J Impot Res. https://doi.org/10.1038/ijir.2013.18

  29. Teixeira da Silva JA (2014) Organogenesis from chrysanthemum Dendranthema x grandiflora (Ramat.) Kitamura petals (disc and ray florets) induced by plant growth regulators. AsPac J Mol Biol Biotechnol 22:145–151

  30. Thomas DT (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631

  31. Usman MMR, Salgar SU, Salkar RJ, Sali LP, Jain NP, Gadgoli CH, Patil PJ (2012) High performance thin layer chromatographic method for quantification of b-sitosterol from Vanda roxburghii R. Br Asian J Plant Sci Res 2:524–529

  32. Van Waes J (1987) Effect of activated charcoal on in vitro propagation of western European orchids. Acta Hortic 212:131–138

  33. Vanneste S, Friml J (2009) Auxin: a trigger for change in plant development. Cell 136:1005–1016

  34. Vij SP, Pathak P (2012) Orchid diversity: conservation and utilization. Proc Natl Acad Sci India Sect B Biol Sci 82:295–300

  35. Wiesman Z, Riov J, Epstein E (1989) Characterization and rooting ability of indole-3-butyric acid conjugates formed during rooting of mung bean cuttings. Plant Physiol 91:1080–1084

  36. Woodward AW, Bartel B (2005) Auxin: regulation, action and interaction. Ann Bot 95:707–735

  37. Zahara M, Datta A, Boonkorkaew P, Mishra A (2017) The effects of different media, sucrose concentrations and natural additives on plantlet growth of Phalaenopsis hybrid ‘Pink.’ Braz Arch Biol Technol 60:1–15


  38.  


Acknowledgements :



The authors are grateful to the Science and Engineering Research Board, Department of Science and Technology, New Delhi, Government of India, for providing financial support as Extra Mural Research Project (EMR/2016/007795, dated 23-08-2017) to their laboratory.


Author Information:



M. Manokari
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, India




Pdf Download