VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 34, Issue: 2, June 2021


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 274

Doi: 10.1007/s42535-021-00211-3
Doi Link: https://doi.org/10.1007/s42535-021-00211-3
First Page: 477
Last Page: 484
Published: 06 April, 2021

Phenolics compounds, evaluation of Alpha-amylase, alpha‐glucosidase inhibitory capacity and antioxidant effect from Globularia alypum L


Abstract:

The aim of this study was to evaluate in vitro antioxidant and inhibitory Alpha-amylase and Alpha-glucosidase activities of fractions from Globularia alypum. Phenolic and flavonoids contents were estimated by Folin–Ciocalteu and aluminum chloride colorimetric methods, respectively. Identification of phenolic constituents was carried out by RP-HPLC–PDA method for diethyl ether and ethyl acetate fractions. Antioxidant activity was assessed by DPPH, FRAP and antihemolysis assays. Antidiabetic activity was evaluated as the first report on in vitro inhibitory effect of fractions on key diabetes enzymes: Alpha-amylase and Alpha-glucosidase. Fractions from G. alypum revealed high enzyme inhibitory activities and potent antioxidant effect. They showed significant antihemolytic results (HT50 from 194 ± 4.86 to 289 ± 5.67 min). Diethyl ether fraction presented the highest amounts in phenolic (331.88 ± 17.80 µg GAE/mg DF) and flavonoids (223.46 ± 2.37 µg CE/mg DF). HPLC analysis revealed the presence of gallic acid, p-coumaric acid, rutin, naringenin and quercetin. It exhibited the highest antioxidant activity assayed by DPPH (IC50 = 20.54 ± 0.48 µg/ml) and FRAP (EC50 = 283.7 ± 82.59 µg/ml). It showed the best inhibitory effect on Alpha-amylase (IC50 = 0.57 ± 0.05 mg/ml) and Alpha-glucosidase (IC50 = 0.52 ± 0.02 mg/ml). Globularia alypum is rich in phenolics and flavonoids. It is a promising source of potent natural antioxidants. It is suggested as an effective plant material for antidiabetic drugs.

Vegetos

Keywords:


n Globularia alypumn , Phenolics compounds, Oxidative stress, Diabetes mellitus, RP-HPLC-PDA


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Acknowledgements :



This research was supported by the Algerian Ministry of Higher Education and Scientific Research (MERS) and General directorate of scientific research and technological development (DGRSDT).


Author Information:



Khaoula Ouffai
Laboratoire Antibiotiques Antifongiques : Physico-chimie, Synthèse et Activité Biologique, Faculté des sciences de la nature et de la vie et des sciences de la terre et de l’univers, Université de Tlemcen, Tlemcen, Algérie




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