VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 34, Issue: 3, September 2021


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 230

Doi: 10.1007/s42535-021-00225-x
Doi Link: https://doi.org/10.1007/s42535-021-00225-x
First Page: 485
Last Page: 494
Published: 10 June, 2021

Medicinal plant assisted cultivation of Pleurotus florida using different lignocellulosic waste substrates


Abstract:

Mushrooms are prominent umbrella-shaped fruiting body of macro fungi known to be highly medicinal, nutritious and tasty; possessing extensive enzyme complexes, which enable them to degrade different lignocellulosic (linin and cellulose) materials. The present study evaluated the growth performance of Oyster mushroom (Pleurotus florida) on five different lingocellulosic wastes such as water hyacinth, rice straw, saw dust, paper and sugar cane bagasse. This study also determined the effects of medicinal leaves (Radish horse drum stick leaf (Moringa oleifera), African basil (Ocimum basilium) and Bitter leaf (Vernonia amygdalina) on growth and nutritional contents of the mushrooms. The mushroom substrates were sterilized, inoculated with actively growing spawn of P. florida and incubated under suitable conditions in a dark room for 3 weeks. The harvested mushrooms were determined for growth parameters such as cap size, stipe, girth and weight. Proximate analysis of mushrooms was carried out for crude protein, crude fat, crude fiber, ash, and moisture content using the guidelines of the Association of Official Analytical Chemist. Growth parameters and proximate analysis data were subjected to simple descriptive and inferential statistics. Results showed that the cultivation cycle of sugar cane bagasse was the shortest (39 days) compared to the other four lignocellulosic substrates. Rice straw gave the highest yield of P. florida for all the harvested flushes with an average weight of 54.1 ± 10.2 g. For supplemented rice straw, the highest yield was observed on rice straw supplemented with African basil with value of 211.68 g and biological efficiency of 105.84%. The study revealed that medicinal leaves could be used to increase the yield of P. florida and influence availability of mushroom throughout the seasons.

Vegetos

Keywords:


Mushroom, Yield, Substrate, Cultivation, Lignocellulosic wastes, Medicinal plants


References:


  1. AOAC (2005) Determination of Moisture, Ash, Protein and Fat. Official Method of Analysis of the Association of Analytical Chemists. 18th Edition, AOAC, Washington DC

  2. Babar I, Hakim KS, Imran K, Bismillah S, Ahmad N, Waseem U, Nangial K, Muhammad A, Syed RAS, Khwaja J, Nazeer A, Mazhar I (2016) Substrates evaluation for the quality, production and growth of oyster mushroom (Pleurotus florida Cetto). J Entomol Zool Stud 4(3):98–107

  3. Birhanli E, Yeşilada O (2013) The utilization of lignocellulosic wastes for laccase production under semisolid-state and submerged fermentation conditions. Turk J Biol 37(4):450–456

  4. Biswas KM, Biswas BS (2015) Recycling of ligno-cellulosic waste materials through oyster mushroom cultivation for sustainable food production. The Ecoscan 9(3–4):655–659

  5. Chandra PP, Neelam K, Usha B, Ram K, Yadav P (2013) Cultivation of Pleurotus sajor-caju using different agricultural residues. Int J Agric Pol Res 1(2):19–23

  6. Çoşkuner Y, Özdemir Y (2000) Acid and EDTA blanching effects on the essential element content of mushrooms (Agaricus bisporus). J Sci Food Agric 80(14):2074–2076

  7. Dehariya P, Vyas D (2013) Effect of different agrowaste substrates and their combinations on the yield and biological efficiency of Pleurotus sajor-caju. J Pharm Biol Sci 8(3):60–64

  8. Diez V, Alvarez A (2001) Composition and nutritional studies on two wild edible mushrooms from northwest Spain. Food Chem 75:417–422

  9. Elansary HO, Norrie J, Ali HM, Salem MZ, Mahmoud EA, Yessoufou K (2016) Enhancement of Calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts. BMC Complement Alter Med 16(1):1–11

  10. Felix GS, Emerson TM, Romildo S, Eustáquio SD (2011) Cultivation of Pleurotus sajorcaju on banana stalk and Bahia grass based substrates. Horticult Bras 29:2

  11. Freedman M, Henika PR, Mandrell RE (2002) Bactericidal activities of plant essential oils and some of their isolated constituents against Campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Protect 65:1545–1560

  12. Grimm D, Wösten HA (2018) Mushroom cultivation in the circular economy. Appl Microbiol Biotechnol 102(18):7795–7803

  13. Hassan F, Medany GM (2014) Effect of pre-treatments and drying temperatures on the quality of dried Pleurotus mushroom SPP. Egypt J Agric Res 92:1009–1023

  14. Hoa HT, Wang CL, Wang CH (2015) The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology 43(4):423–434

  15. Inam-ul-Haq M, Khan NA, Khan MA, Khan MA, Javed N, Binyamin R, Irshad G (2010) Use of medicinal plants in different composts for yield improvement of various strains of oyster mushroom. Pak J Bot 42(5):3275–3283

  16. Ingale A, Ramteke A (2010) Studies on cultivation and biological efficiency of mushrooms grown on different agro-residues. Rom J Food Biotechnol 6:25–28

  17. Khare KB, Kihumbu AG, Shitandi AA, Mahungu MS, Harish KS (2008) Nutritional composition of Pleurotus sajor-caju grown on water hyacinth, wheat straw and corn cob substrates. Res J Agric Biol Sci 4:291–297

  18. Kulanthaivel S, Tanya Susan G, Narasinganallur SV, Kilavan PK (2011) Ehanol production from lignocellulosic waste. Department of Biotechnology, BannariAmman Institute of Technology, Sathyamangalam. World J Sci Technol 1(11):2231–2587

  19. Kumar S, Chand G, Patel DK (2020) Evaluation of different substrate supplements on growth and yield of oyster mushroom (Pleurotus florida). Indian Phytopathol 73:731–736

  20. Lalithadevy V, Many JN (2014) Yield performance of fruits and vegetables peel as substrates for cultivation of oyster mushroom (Pleurotus florida). J Innov Res Sol 1(1):220–226

  21. Manzi P, Aguzzi A, Pizzoferrato L (2001) Nutritional value of mushrooms widely consumed in Italy. Food Chem 73:321–325

  22. Mattila P, Salo Väänänen P, Könkö K, Aro H, Jalava T (2002) Basic composition and amino acid contents of mushrooms cultivated in Finland. J Agric Food Chem 50:6419–6422

  23. Mehta V, Gupta JK, Kaushal SC (1990) Cultivation of Pleurotus florida mushroom on rice straw and biogas production from the spent straw. World J Microbiol Biotechnol 6:366–370

  24. Miles PG, Chang ST (2004) Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact. CRC Press, pp 4–6

  25. Moda EM, Horii J, Spoto MHF (2005) Edible fungus Pleurotus sajor-caju production on washed and supplemented sugarcane bagasse. Sci Agric 62:127–132

  26. Muhammad UC, Imran K, Muhammad UH, Muhammad BC, Muhammad N, Asif I, Nazar HK, Naveed A, Muhammad U, Mina KM, Aman U (2018) Efficacy of extraction methods of Moringa oleifera leaf extract for enhanced growth and yield of wheat. Basic Appl Sci 14:131–135

  27. Naraian R, Dixit B (2017) Nutritional value of three different oyster mushrooms grown on cattail weed substrate. Arch Biotechnol Biomed 1:61–66

  28. Nwachukwu EO, Umechuruba CI (2001) Antifungal activities of some leaf extracts on seed-borne fungi of African yam bean seeds, seed germination and seedling emergence. Appl Sci Environ Manag 5(1):29–32

  29. Oyedele OA, Adeosun MV, Koyenikan OO (2018) Low cost production of mushroom using agricultural waste in a controlled environment for economic advancement. Int J Waste Res 8:329–333

  30. Prasad S, Rathore H, Sharma S, Yadav AS, Naik SN (2017) Studies on effects of supercritical CO2 extraction on yield and antioxidant activity of Ledodes extract. Res J Pharma Biol Chem Sci 80:1144–1154

  31. Saini JK, Saini R, Tewari L (2015) Lignocellulosic agriculture wastes as biomass feedstocks for second-generation bioethanol production: concepts and recent developments. 3 Biotech 5(4):337–353

  32. Shivabasu K, Mallesha BC (2017) Effect of different substrates for higher bioefficiency of mushrooms. Int J Curr Microbiol Appl Sci 6(6):3113–3312

  33. Silveira MLL, Furlan SA, Ninow JL (2008) Development of an alternative technology for the oyster mushroom production using liquid inoculum. J Food Sci Technol 28(4):858–862

  34. Sun SJ, Liu JZ, Hu KH, Zhu HX (2011) The level of secreted laccase activity in the edible fungi and their growing cycles are closely related. Curr Microbiol 62(3):871–875

  35. Taha M, Foda M, Shahsavari E, Aburto-Medina A, Adetutu EM, Ball AS (2016) Commercial feasibility of lignocellulose biodegradation: possibilities and challenges. Curr Opin Biotechnol 38:190–197

  36. Teklit GA (2015) Chemical composition and nutritional value of the most widely used mushrooms cultivated in Mekelle Tigray Ethiopia. J Nutr Food Sci 5:408

  37. Tolera DK, Abera S (2017) Nutritional quality of Oyster Mushroom (Pleurotus ostreatus) as affected by osmotic pretreatments and drying methods. Food Sci Nutr 5:989–996

  38. Usha RP, Ram C, Pushpendra KD (2018) Comparative study on growth parameters and yield potential of two species of pleurotus mushroom (Pleurotus florida and Pleurotus sajor-caju). Int J Curr Microbiol Appl Sci 7(3):3066–3071

  39. Vidya JT, Sobita S, Abhilasha AL (2017) Efficacy of different substrates on the growth, yield and nutritional composition of oyster mushroom-Pleurotus florida (Mont.) Singer. J Pharmacog Phytochem 6(4):1097–1100


  40.  


Acknowledgements :




Author Information:



Tolulope T. Mapayi
Institute of Ecology and Environmental Studies, Obafemi Awolowo University, Ile-Ife, Nigeria

Olumide O. Omoboye
Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria

Adewale M. Taiwo
Department of Environmental Management and Toxicology,Federal University of Agriculture, Abeokuta, Nigeria

taiwoademat2003@yahoo.com




Pdf Download