VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 34, Issue: 3, September 2021


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 66

Doi: 10.1007/s42535-021-00251-9
Doi Link: https://doi.org/10.1007/s42535-021-00251-9
First Page: 600
Last Page: 605
Published: 30 June, 2021

Citrus essential oils and main terpenes: chemical composition and good litholytic activity on gallstones


Abstract:

We studied the gallstone dissolving capacity of Moroccan citrus (C) essential oils; C. clementina, C. aurantium, C. sinensis and C. paradisi. Their litholytic activity was compared to that of their main constituents revealed by a gas chromatography-mass spectrometry (GC–MS) analysis; limonene, linalool and γ-terpinene and to the Methyl tertbutyl ether (MTBE) standard as well. Gallstone sample was classified as a cholesterol type after a quantification analysis, using UV-spectrophotometer. The hydrocarbon compounds predominated the chemical composition of the oils, limonene was the commonly major identified compound, representing between 72.06 and 88.49 %. The citrus essential oils caused a deterioration in the morphological appearance of gallstone samples. The C. sinensis essential oil manifested with remarkable litholytic activity after a day of incubation, reached to a dissolution capacity of 95.78 ± 0.49 %, the same for C. clementina with 94.81 ± 1.30 % and C. aurantium with 92.99 ± 2.04 %. These oils were more effective than MTBE standard. In last position, C. paradisi essential oil exhibited the less high dissolution of 87.68 ± 1.82 %, comparing with other oils. Linalool showed a good litholytic activity, better than that of MTBE and similar to that of limonene. Therefore, in addition to limonene role, also the other constituents may have, as a natural mixture, an important additive effect in the gallstone dissolution of each essential oil.

Vegetos

Keywords:


Moroccan citrus fruits, Essential oil, Terpenes, Gallstones, Litholytic activity


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Acknowledgements :



We would like to thank Professor Ibrahim Sbai El Otmani from Faculty of Medicine and Pharmacy Casablanca, Hassan II University of Casablanca for enthusiastic help.


Author Information:



Asma Arrout
Laboratory of Drugs Sciences, Biomedical Research and Biotechnology, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca, Morocco

Mohammed Rachid Lefriyekh
Department General Surgery, Faculty of Medicine and Pharmacy, University Hospital Center Ibn Rochd, Hassan II University of Casablanca, Casablanca, Morocco

Amal Ait Haj Said
Laboratory of Drugs Sciences, Biomedical Research and Biotechnology, Faculty of Medicine and Pharmacy, Hassan II University of Casablanca, Casablanca, Morocco




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