VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 4, December 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 240

Doi: 10.1007/s42535-020-00154-1
Doi Link: https://doi.org/10.1007/s42535-020-00154-1
First Page: 658
Last Page: 664
Published: 03 September, 2020

Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb


Abstract:

An efficient in vitro propagation protocol for an important medicinal endangered herb Ferula jaeschkeana has been developed through somatic embryogenesis. 12 week old friable calli were derived from petiole explants on a medium supplemented with 2.0 mgl−1 2,4-D and 1.0 mgl−1 Kn and used as mother source for somatic embryogenesis. Different auxins (2,4-D, IBA, IAA and NAA) in different concentrations (0.2–4.0 mgl−1) was used to produce embryogenic callus. Eighty four percent calli produced globular embryos on a medium supplemented with 4.0 mgl−1 2,4-D. The globular embryo then mature on culture medium (Control) or supplemented with different addictives (auxin, proline and ABA), maturation was significantly affected by ABA, proline and number of subcultures with maximum number of mature embryo (32.1 ± 0.2) was formed on medium fortified with 2.0 mgl−1 proline. Mature embryos had two well-developed cotyledons and an elongated hypocotyl root axis. 78% of these embryos were transformed into plantlets when placed in culture medium fortified with 0.2 + 0.01 + 0.05 mgl−1 (BAP:IBA:GA3) respectively. Regenerated plants were hardened in vermiculite with a 23% survival rate. The regenerated plants showed normal diploid chromosome number (2n = 22) and leaf morphology was comparable to in vivo plant.

Vegetos

Keywords:


Petiole, Callus, Somatic embryos, Abscisic acid, Proline, Cytology


References:


  1. Ammirato PV (1974) The effects of abscisic acid on the development of somatic embryos from cells of caraway (Carum carvi L.). Bot Gaz 135:328–337

  2. Barna KS, Wakhlu AK (1993) Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Rep 12:521–524

  3. Bennici A, Anzidei M, Vendramin GG (2004) Genetic stability and uniformity of Foeniculum vulgare Mill. regenerated plants through organogenesis and somatic embryogenesis. Plant Sci 166:221–227

  4. Bernard F, Bazarnov HS, Khatab LJ, Darbi AS, Sheidai M (2007) Ferula gummosa Boiss. Embryogenic culture and karyological changes. Pak J Biol Sci 10:1977–1983

  5. Dawa S, Gurmet P, Dolma T, Angdus T, Stobgais T, Tharpa T (2018) Status of medicinal and aromatic plants in the state of Jammu and Kashmir, India. Int J Curr Microbiol App Sci 7:2597–2615

  6. Dubey NK, Kumar R, Tripathi P (2004) Global promotion of herbal medicine: India's opportunity. Curr Sci 86:37–41

  7. Fujimura T, Komamine A (1975) Effect of various growth regulators on the embryogenesis in carrot cell suspension cultures. Plant Sci Lett 5:13–19

  8. Hadi N, Moeini A, Omidbaigi R (2011b) Responses of zygotic embryos of galbanum in in vitro conditions. Planta Med. https://doi.org/10.1055/s-0031-1282302

  9. Hadi N, Omidbaigi R, Moeinei A (2011a) In vitro conservation of Ferula gummosa Boiss. and its callus induction. Iran J Rangelands Forests Plant Bree Genetic Res 19:28–38

  10. Hasani B, Saboura A, Rajabian T, Falah HH (2008) Somatic embryogenesis of Ferula assa-foetida L. JUST 33:15–23

  11. Hirai G, Kasai N, Harada T (1997) Somatic embryogenesis in mature zygotic embryo culture of Glehnia littoralis. Plant Cell Tiss Org Cult 48:175–180

  12. Hiraoka N, Oyanagi M (1992) Micropropagation of Glehnia (Glehnia littoralis Fr. Schmidt ex Miq.). In High-Tech and Micropropagation III. Springer, Berlin, pp 443–452

  13. Huber J, Constabel F, Gamborg OL (1978) A cell counting procedure applied to embryogenesis in cell suspension cultures of anise (Pimpinella anisum L.). Plant Sci Lett 12:209–215

  14. Hunault G, Desmarkt P (1990) Field assessment of somaclonal variation during somatic embryogenesis in bitter Fennel. Bull Soc Bot Fr Actual Bot 137:45–49

  15. Hussain A, Virmani OP, Sharma A, Kumar A, Misra LN (1988) Major essential oil bearing plants in India. Central Institute of Medicinal and Aromatic Plants, Lucknow

  16. Khajuria AK, Bisht NS, Bhagat N (2020) In vitro organogenesis and plant regeneration of Thymus serpyllum L.: an important aromatic medicinal plant. Vitro Cell Dev Bio plant. https://doi.org/10.1007/s11627-020-10094-9

  17. Khajuria AK, Chandra S, Manhas RK, Bisht NS (2019) Effect of different PGRs on in vitro organogenesis in Viola canescens Wall. Ex. Roxb from petiole callus culture. Vegetos 32:353–362

  18. Khalilova ÉK, Saidkhodzhaev AI (1998) Terpenoid coumarins of Ferula sumbul. Chem Nat Comp 34:506–507

  19. Khare CP (2008) Indian medicinal plants: an illustrated dictionary. Springer Science & Business Media, Berlin

  20. Kim SW, Park MK, Liu JR (1996) High frequency plant regeneration via somatic embryogenesis in cell suspension cultures of coriander (Coriandrun sativum L.). Plant Cell Rep 15:751–753

  21. Komatsuda T, Lee W, Oka S (1992) Maturation and germination of somatic embryos as affected by sucrose and plant growth regulators in soyabeans Glycine gracilis Skvortz and Glycine max (L.) Men. Plant Cell Tiss Org Cult 28:103–113

  22. Mabberley DJ (2008) Mabberley’s plant book: a portable dictionary of plants, 3rd edn. Cambridge University Press, Cambridge

  23. Mohammadhosseinia M, Vendittib A, Sarkerc SD, Naharc L, Akbarzadehd A (2019) The genus Ferula: ethnobotany, phytochemistry and bioactivities—a review. Ind Crops Prod 129:350–394

  24. Nickle TC, Yeung EC (1993) Failure to establish a functional shoot meristem may be because of concersion failure in somatic embryos of Daucus carota (Apiaceae). Am J Bot 80:1284–1291

  25. Otroshy M, Edrisi S, Enteshary S (2013) Propagation of medicinal plant Ferula assafoetida L. through indirect somatic embryogenesis. Int J Med Plant Res 2:179–186

  26. Otroshy M, Roozbeh S (2015) Optimization of somatic embryogenesis in Ferula assafoetida L. (a rare medicinal plant). Annu Res Rev Biol 5:439–448

  27. Paul S, Samant SS, Lal M, Sharma L, Ram J (2019) Population status and habitat modelling of high value vulnerable Ferula jaeschkeana for conservation in North-Western Himalaya. Med Plants 11:117–126

  28. Pimenov MG, Leonov MV (2004) The Asian Umbelliferae biodiversity database (ASIUM) with particular reference to south-west Asian taxa. Turk J Bot 28:139–145

  29. Rajesh M, Sivanandhan G, Subramanyam K, Kapildev G, Jaganath B, Kasthurirengan S, Ganapathi A (2014) Establishment of somatic embryogenesis and podophyllotoxin production in liquid shake cultures of Podophyllum hexandrum Royle. Ind Crops Prod 60:66–74

  30. Revathi J, Manokari M, Latha R, Priyadharshini S, Kher MM, Shekhawat MS (2019) In vitro propagation, in vitro flowering, ex vitro root regeneration and foliar micro-morphological analysis of Hedyotis biflora (Linn.) Lam. Vegetos 32:609–619

  31. Salehia M, Naghavia MR, Bahmankarb M (2019) A review of Ferula species: biochemical characteristics, pharmaceutical and industrial applications, and suggestions for biotechnologists. Ind Crops Prod 139:111511

  32. Samant SS, Dhar U, Palni LMS (1998) Medicinal plants of Indian Himalaya: diversity distribution potential value. Gyanodaya Prakashan, Nainital, pp 155–158

  33. Samant SS, Pant S, Singh M, Lal M, Singh A, Sharma A, Bhandari S (2007) Diversity, distribution pattern, indigenous uses and conservation prioritization of medicinal plants of Himachal Pradesh, India. Int J Biodiver Sci Manage 3:234–251

  34. Sharma RK (2015) Callus formation in Ferula jaeschkeana vatke. Int Plant Sci 10:98–101

  35. Sharma RK, Wakhlu AK (1998) Somatic embryogenesis and plant regeneration in Heracleum candicans Wall. Plant Cell Rep 17:866–869

  36. Singh MM, Agnihotri A, Garg SN, Agarwal SK, Guptat DN, Keshri U, Kamboj VP (1987) Antifertility and hormonal properties of certain carotane sesquiterpenes of Ferula jaeschkeana. Planta Med. 54:494–494

  37. Singh R, Rai MK, Kumari N (2015) Somatic embryogenesis and plant regeneration in Sapindus mukorossi Gaertn. from leaf-derived callus induced with 6-benzylaminopurine. Appl Biochem Biotech 177:498–510

  38. Smith DL, Krikorian AD (1988) Production of somatic embryos from carrot tissue in hormone free medium. Plant Sci 58:103–110

  39. Suran D, Bolor T, Bayarmaa GA (2016) In vitro seed germination and callus induction of Ferula ferulaeoides (Steud.) Korov. (Apiaceae). Mong J Biol Sci 14:53–58

  40. Te-chato S, Hilae A (2007) High-frequency plant regeneration through secondary somatic embryogenesis in oil palm (Elaeis guineensis Jacq. Var. tenera). J Agricult Technology 3:345–357

  41. Tuncer B (2017) Investigation of the in vitro regeneneration of some medicinal and aromatic wild plant species. APP Ecol Env Res 15:905–914

  42. Vandemoortele JL, Billard JP, Boucaud J, Gaspar TH (1996) Micropropagation of Parsley shoot proliferation. Plant Tiss Org Cult 44:25–30

  43. Vasil IK (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops. J Plant Physiol 128:193–218

  44. Vasil IK, Hildebrandt AC (1966) Variations of morphogenetic behavior in plant tissue cultures II. Petroselinum hortense. Am J Bot 53:869–874

  45. Wakhlu AK, Nagari S, Barna KS (1990) Somatic embryogenesis and plant regeneration from callus cultures of Bunium persicum Boiss. Plant Cell Rep 9:137–138

  46. Wakhlu AK, Sharma RK (1998) Somatic embryogenesis and plant regeneration in Heracleum candicans wall. Plant Cell Rep 17:866–869

  47. Williams L, Collin HA (1976) Somatic embryogenesis and plantlet formation in tissue culture of Celery. Ann Bot 40:325–332

  48. Wochok ZS, Wetherell DF (1971) Suppression of organized growth in cultured wild carrot tissue by 2-chloroethylphosphoric acid Physiol. Tokyo 12:771–774

  49. Yaqoob U, Nawchoo IA (2016) Distribution and taxonomy of Ferula L.: a review. Res Rev J Bot 5:15–23

  50. Yaqoob U, Nawchoo IA (2017a) a) Conservation and cultivation of Ferula jaeschkeana Vatke: a species with deep complex morphophysiological dormancy. Proc Natl Acad Sci India B 87:315–325

  51. Yaqoob U, Nawchoo IA (2017b) Impact of habitat variability and altitude on growth dynamics and reproductive allocation in Ferula jaeschkeana Vatke. J King Saud Univ Sci 29:19–27

  52. Yumbla-Orbes M, Rocha DI, Matos EM, Koehler AD, Pinheiro MVM, Batista DS, Freitas DMS, Cruz ACF, Barbosa JG, Viccini LF, Otoni WC (2020) Somatic embryogenesis induced from vascular tissues in leaf explants of Lisianthus (Eustoma grandiflorum (Raf.) Shinn) generates true-to-type diploid plants. Vegetos 33:135–144

  53. Zare AR, Solouki M, Omidi M, Irvani N, Mahdi NN, Rezazadeh S (2010) Callus induction and plant regeneration in ferula assa Foetida L. (Asafetida), an endangered medicinal plant. Trakia J Sci 8:11–18


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Acknowledgements :



The authors gratefully acknowledge the department for providing facilities.


Author Information:



R. K. Sharma
Department of Botany, School of Sciences, Cluster University of Jammu, Jammu, India

Arun Kumar Khajuria
Department of Botany, School of Sciences, Cluster University of Jammu, Jammu, India
arun.khajuria20@gmail.com




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