VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 4, December 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 58

Doi: 10.1007/s42535-020-00155-0
Doi Link: https://doi.org/10.1007/s42535-020-00155-0
First Page: 665
Last Page: 681
Published: 03 September, 2020

Exogenous trehalose ameliorates methyl viologen induced oxidative stress through regulation of stomatal pore opening and glutathione metabolism in tomato seedlings


Abstract:

Oxidative burst is the common thread linking all abiotic and biotic stressors. So in this study non-reducing disaccharide trehalose (Tre) has been examined for attenuation of oxidative stress, imposed through methyl viologen (MV) foliar spray in tomato seedlings. Initially the ameliorating property was ascertained by leaf disc senescence and stomatal pore opening assay, before carrying out the foliar spray on seedlings. Both these assays showed Tre mediated mitigation of MV induced oxidative stress. It was also observed that trehalose dose determines the extent of stomatal pore opening. MV treated seedlings showed more reactive oxygen species (ROS) accumulation compared to control, Tre and MV + Tre treated plants both histochemically and quantitatively. Damage due to ROS accumulation was documented by quantifying malondialdehye and lipoxygenase activity. Finally, the response of the tomato seedlings to oxidative stress was monitored by quantifying the antioxidant enzyme activities and non-enzymatic antioxidant content under stressed and unstressed conditions. Also transcript levels of glutathione synthetase (GS) and gamma-glutamylcysteine synthetase (γ-ECS), enzymes responsible for GSH synthesis; and transcription factors no apical meristem ATAF and cup-shaped cotyledon (NAC2) and dehydration-responsive element-binding (DREB) were found to increase on application of Tre. All of these experimentations showed better adaptability and survivability of the seedlings treated exogenously with both MV and Tre in comparison to those treated only with MV.

Vegetos

Keywords:


Trehalose, Methyl viologen, Oxidative stress, Tomato


References:


  1. Aarti PD, Tanaka R, Tanaka A (2006) Effects of oxidative stress on chlorophyll biosynthesis in cucumber (Cucumis sativus) cotyledons. Physiol Plant 128(1):186–197

  2. Ali Q, Ashraf M (2011) Induction of drought tolerance in maize (Zea mays L.) due to exogenous application of trehalose: growth, photosynthesis, water relations and oxidative defence mechanism. J Agron Crop Sci 197(4):258–271

  3. Anderson ME (1985) [70] Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 113:548–555

  4. Anjum NA, Ahamad I, Mohmood I, Pacheco M, Duarte A, Pereira E, Umar S, Ahamad A, Khan NA, Iqbal M, Prasad MNV (2012) Modulationof glutathione and its related enzymes in plants responses to toxic metals and metalloids—a review. Environ Exp Bot 75:307–324

  5. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1

  6.  

  7. Barnett NM, Naylor AW (1966) Amino acid and protein metabolism in Bermuda grass during water stress. Plant Physiol 41(7):1222–1230

  8. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205–207

  9. Borgohain P, Saha B, Agrahari R, Chowardhara B, Sahoo S, van der Vyver C, Panda SK (2019) SlNAC2 overexpression in Arabidopsis results in enhanced abiotic stress tolerance with alteration in glutathione metabolism. Protoplasma 256(4):1065–1077

  10.  

  11. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254

  12. Bray EA, Bailey-Serres JW, Weretilnyk E (2000) Responses to abiotic stresses. Biochem Mol Biol Plants, 1158–1203

  13. Chance B, Maehly AC (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775

  14. Chang B, Yang L, Cong W, Zu Y, Tang Z (2014) The improved resistance to high salinity induced by trehalose is associated with ionic regulation and osmotic adjustment in Catharanthus roseus. Plant Physiol Biochem 77:140–148

  15.  

  16. Choudhury S, Panda P, Sahoo L, Panda SK (2013) Reactive oxygen species signaling in plants under abiotic stress. Plant Signal Behav 8(4):e23681

  17. Dalal A, Vishwakarma A, Singh NK, Gudla T, Bhattacharyya MK, Padmasree K, Kirti PB (2014) Attenuation of hydrogen peroxide-mediated oxidative stress by Brassica juncea annexin-3 counteracts thiol-specific antioxidant (TSA1) deficiency in Saccharomyces cerevisiae. FEBS Lett 588(4):584–593

  18.  

  19. Dar MI, Naikoo MI, Rehman F, Naushin F, Khan FA (2016) Proline accumulation in plants: roles in stress tolerance and plant development. In: Osmolytes and plants acclimation to changing environment: emerging omics technologies. Springer, New Delhi, pp 155–166

  20. Donahue JL, Okpodu CM, Cramer CL, Grabau EA, Alscher RG (1997) Responses of antioxidants to paraquat in pea leaves (relationships to resistance). Plant Physiol 113(1):249–257

  21. Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28(3):350–356

  22. Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxyl ammonium chloride: a simple assay for superoxide dismutase. Anal Biochem 70(2):616–620

  23.  

  24.  

  25. Fernandez O, Béthencourt L, Quero A, Sangwan RS, Clément C (2010) Trehalose and plant stress responses: friend or foe? Trends Plant Sci 15(7):409–417

  26. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48(12):909–930

  27. Gupta AS, Heinen JL, Holaday AS, Burke JJ, Allen RD (1993) Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci 90(4):1629–1633

  28. Habig WH, Jakoby WB (1981) Assays for differentiation of glutathione S-transferases. Methods Enzymol 77:398–405

  29. Harrach BD, Fodor J, Pogány M, Preuss J, Barna B (2008) Antioxidant, ethylene and membrane leakage responses to powdery mildew infection of near-isogenic barley lines with various types of resistance. Eur J Plant Pathol 121(1):21–33

  30. Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Biol 51(1):463–499

  31. Havé M, Leitao L, Bagard M, Castell JF, Repellin A (2015) Protein carbonylation during natural leaf senescence in winter wheat, as probed by fluorescein-5-thiosemicarbazide. Plant Biol 17(5):973–979

  32. Hazarika P, Rajam MV (2011) Biotic and abiotic stress tolerance in transgenic tomatoes by constitutive expression of S-adenosylmethionine decarboxylase gene. Physiol Mol Biol Plants 17(2):115–128

  33. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198

  34. Husen A, Iqbal M, Aref IM (2014) Growth, water status, and leaf characteristics of Brassica carinata under drought and rehydration conditions. Braz J Bot 37(3):217–227

  35. Kar M, Mishra D (1976) Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiol 57(2):315–319

  36. Lee YP, Kim SH, Bang JW, Lee HS, Kwak SS, Kwon SY (2007) Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. Plant Cell Rep 26(5):591–598

  37. Lemichez E, Wu Y, Sanchez JP, Mettouchi A, Mathur J, Chua NH (2001) Inactivation of AtRac1 by abscisic acid is essential for stomatal closure. Genes Dev 15(14):1808–1816

  38. Levine RL, Williams JA, Stadtman EP, Shacter E (1994) [37] Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233:346–357

  39.  

  40. Liu C, Liu Y, Guo K, Fan D, Li G, Zheng Y, Yang R (2011) Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. Environ Exp Bot 71(2):174–183

  41. Lobell DB, Schlenker W, Costa-Roberts J (2011) Climate trends and global crop production since 1980. Science 333(6042):616–620

  42. Márquez-Escalante JA, Figueroa-Soto CG, Valenzuela-Soto EM (2006) Isolation and partial characterization of trehalose 6-phosphate synthase aggregates from Selaginella lepidophylla plants. Biochimie 88(10):1505–1510

  43. Mishra S, Kumar S, Saha B, Awasthi J, Dey M, Panda SK, Sahoo L (2016) Crosstalk between salt, drought, and cold stress in plants: toward genetic engineering for stress tolerance. Abiotic stress response in plants

  44. Mostofa MG, Hossain MA, Fujita M, Tran LSP (2015) Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice. Sci Rep 5:11433

  45. Murchie EH, Lawson T (2013) Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot 64(13):3983–3998

  46. Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22(5):867–880

  47. Oser BL (1979) Hawks physiological chemistry. McGraw Hill, New York, pp 702–705. ISBN 0-07-099490-0

  48. Paul MJ, Primavesi LF, Jhurreea D, Zhang Y (2008) Trehalose metabolism and signaling. Annu Rev Plant Biol 59:417–441

  49. Porta H, Rocha-Sosa M (2002) Plant lipoxygenases. Physiological and molecular features. Plant Physiol 130(1):15–21

  50. Ramel F, Sulmon C, Bogard M, Couée I, Gouesbet G (2009) Differential patterns of reactive oxygen species and antioxidative mechanisms during atrazine injury and sucrose-induced tolerance in Arabidopsis thaliana plantlets. BMC Plant Biol 9(1):28

  51. Rao MV, Davis KR (1999) Ozone-induced cell death occurs via two distinct mechanisms in Arabidopsis: the role of salicylic acid. Plant J 17(6):603–614

  52. Sagisaka S (1976) The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol 57(2):308–309

  53. Saha B, Borovskii G, Panda SK (2016a) Alternative oxidase and plant stress tolerance. Plant Signal Behav 11(12):e1256530

  54. Saha B, Mishra S, Awasthi JP, Sahoo L, Panda SK (2016b) Enhanced drought and salinity tolerance in transgenic mustard [Brassica juncea (L.) Czern & Coss.] overexpressing Arabidopsis group 4 late embryogenesis abundant gene (AtLEA4-1). Environ Exp Bot 128:99–111

  55. Sahoo S, Saha B, Awasthi JP, Omisun T, Borgohain P, Hussain S, Panda SK (2019) Physiological introspection into differential drought tolerance in rice cultivars of North East India. Acta Physiol Plant 41(4):53

  56. Shao HB, Chu LY, Jaleel CA, Manivannan P, Panneerselvam R, Shao MA (2009) Understanding water deficit stress-induced changes in the basic metabolism of higher plants—biotechnologically and sustainably improving agriculture and the ecoenvironment in arid regions of the globe. Crit Rev Biotechnol 29(2):131–151

  57. Sheng M, Tang M, Chen H, Yang B, Zhang F, Huang Y (2008) Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18(6–7):287–296

  58. Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5, 5′-dithiobis (2-nitrobenzoic acid). Anal Biochem 175(2):408–413

  59. Sullivan CY (1972) Mechanisms of heat and drought resistance in grain sorghum and methods of measurement. Sorghum in seventies. Oxford and IBH Pub Co, Zhonghua

  60.  

  61. Thomas H, Howarth CJ (2000) Five ways to stay green. J Exp Bot 51(suppl 1):329–337

  62. Valliyodan B, Nguyen HT (2006) Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Curr Opin Plant Biol 9(2):189–195

  63. Van den Ende W, Peshev D (2013) Sugars as antioxidants in plants. In: Crop improvement under adverse conditions. Springer, New York, pp 285–307

  64. Vellosillo T, Martínez M, López MA, Vicente J, Cascón T, Dolan L, Castresana C (2007) Oxylipins produced by the 9-lipoxygenase pathway in Arabidopsis regulate lateral root development and defense responses through a specific signaling cascade. Plant Cell 19(3):831–846

  65. Wang W, Vinocur B, Altman A (2003) Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218(1):1–14

  66. Williams M, Salas JJ, Sanchez J, Harwood JL (2000) Lipoxygenase pathway in olive callus cultures (Olea europaea). Phytochemistry 53:13–19

  67. Xia Q, El-Maarouf-Bouteau H, Bailly C, Meimoun P (2016) Determination of protein carbonylation and proteasome activity in seeds. Plant proteostasis: methods and protocols, pp 205–212


  68.  


Acknowledgements :



PB, BC, JPA acknowledge UGC, India for NON-NET Fellowship.


Author Information:



Pankaj Borgohain
Plant Molecular Biotechnology Lab, Department of Life Science and Bioinformatics, Assam University, Silchar, India

Bhaben Chowardhara
Plant Molecular Biotechnology Lab, Department of Life Science and Bioinformatics, Assam University, Silchar, India


Bedabrata Saha
Plant Molecular Biotechnology Lab, Department of Life Science and Bioinformatics, Assam University, Silchar, India




Pdf Download
Share: