VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 4, December 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 187

Doi: 10.1007/s42535-020-00160-3
Doi Link: https://doi.org/10.1007/s42535-020-00160-3
First Page: 703
Last Page: 711
Published: 09 October, 2020

Antimicrobial activity of Lavandula stoechas phenolic extracts against pathogenic bacteria isolated from a hospital in Morocco


Abstract:

The objective of this study was to determine the chemical families and the total phenolic content in a hydro-ethanolic extract of Lavandula stoechas, as well as to evaluate the antibacterial effect of the different phenolic fractions of this plant against pathogenic bacterial strains isolated from a hospital in the city of Fez, Morocco. The hydro-ethanolic extract was obtained and phytochemical screening of the different chemical families of the plant was conducted using colorimetric methods. The quantification of the total concentration of polyphenols in the L. stoechas extract was conducted according to the Folin–Ciocalteu method. The antimicrobial effects of the hydro-ethanolic, flavonoid and tannin extracts against Gram-positive and Gram-negative pathogenic bacteria isolated from a hospital in Fez were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for all pathogenic and reference strains. The total phenolic content of L. stoechas was 130.15 ± 3.72 mg gallic acid equivalent/g plant extract. The total phenolic content of L. stoechas was 130.15 ± 3.72 mg gallic acid equivalent/g plant extract. The flavonoid extract had the highest effect against bacteria, with MIC and MBC values varying from 10 to 40 mg/ml and 20–80 mg/ml, respectively. The results also revealed that the hydro-ethanolic extract showed a MIC value of 80 mg/ml against all bacteria except Acinetobacter baumanii and Pseudomonas aeruginosa (ATCC27853). The results obtained in our study showed an important antibacterial effect of flavonoid extracts from L. stoechas compared to other extracts. These results can aid in the discovery of new antimicrobial molecules of natural origin. Further research on phenolic compounds in L. stoechas is required.

Vegetos

Keywords:


n Lavandula stoechasn , Ultrasound assisted extraction, Phenolic fractions, Antimicrobial activity, Hospital environment


References:


  1. Aiyegoro OA, Okoh AI (2010) Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern Med 10:21

  2. Alabri THA, Al Musalami AHS, Hossain MA, Weli AM, Al-Riyami Q (2014) Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ Sci 26:237–243

  3. Algieri F, Rodriguez-Nogales A, Vezza T, Garrido-Mesa J, Garrido-Mesa N, Utrilla MP, González-Tejero MR, Casares-Porcel M, Molero-Mesa J, del Mar CM, Segura-Carretero A, Pérez-Palacio J, Diaz C, Vergara N, Vicente F, Rodriguez-Cabezas ME, Galvez J (2016) Anti-inflammatory activity of hydroalcoholic extracts of Lavandula dentata L. and Lavandula stoechas L. J Ethnopharmacol 190:142–158

  4. Al-Niaame AE, Aziz RA (2013) Study of Lavandula officinalis L. buds of flowers extracts activity against some species of multi-drug resistant clinical isolates of bacteria. Iraqi J Biotechnol 12:82–91

  5. Alves MJ, Ferreira ICFR, Froufe HJC, Abreu RMV, Martins A, Pintado M (2013) Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. J Appl Microbiol 115:346–357

  6. Babar PS, Deshmukh AV, Salunkhe SS, Chavan JJ (2020) Micropropagation, polyphenol content and biological properties of Sweet Flag (Acorus calamus): a potent medicinal and aromatic herb. Vegetos 33:296–303

  7. Bajalan I, Mohammadi M, Alaei M, Pirbalouti AG (2016) Total phenolic and flavonoid contents and antioxidant activity of extracts from different populations of lavandin. Ind Crops Prod 87:255–260

  8. Baptista R, Madureira AM, Jorge R, Adão R, Duarte A, Duarte N, Lopes MM, Teixeira G (2015) Antioxidant and Antimycotic Activities of Two Native Lavandula Species from Portugal. https://www.hindawi.com/journals/ecam/2015/570521/. Accessed 8 May 2020

  9. Canlı K, Yetgin A, Benek A, Bozyel ME, Murat Altuner E (2019) In Vitro Antimicrobial Activity Screening of Ethanol Extract of Lavandula stoechas and Investigation of Its Biochemical Composition. https://www.hindawi.com/journals/aps/2019/3201458/. Accessed 8 May 2020

  10. Carling PC, Huang SS (2013) Improving healthcare environmental cleaning and disinfection current and evolving issues. Infect Control Hosp Epidemiol 34:507–513

  11. Celep E, Akyüz S, İnan Y, Yesilada E (2018) Assessment of potential bioavailability of major phenolic compounds in Lavandula stoechas L. ssp. stoechas. Ind Crops Prod 118:111–117

  12. Chen Z, He B, Zhou J, He D, Deng J, Zeng R (2016) Chemical compositions and antibacterial activities of essential oils extracted from Alpinia guilinensis against selected foodborne pathogens. Ind Crops Prod 83:607–613

  13. Choi H-S, Sawamura M, Song H-S (2010) Functional properties. Citrus essential oils. Wiley, New York, pp 229–296

  14. Cushnie TPT, Lamb AJ (2005) Antimicrobial activity of flavonoids. Int J Antimicrob Agents 26:343–356

  15. del Villalobos M, Serradilla MJ, Martín A, Ordiales E, Ruiz-Moyano S, de Córdoba M (2016) Antioxidant and antimicrobial activity of natural phenolic extract from defatted soybean flour by-product for stone fruit postharvest application: Antioxidant and antimicrobial activity. J Sci Food Agric 96:2116–2124

  16. dos Santos C, Vargas Á, Fronza N, dos Santos JHZ (2017) Structural, textural and morphological characteristics of tannins from Acacia mearnsii encapsulated using sol–gel methods: applications as antimicrobial agents. Colloids Surf B Biointerfaces 151:26–33

  17. Edris AE (2007) Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother Res 21:308–323

  18. El-Hilaly J, Hmammouchi M, Lyoussi B (2003) Ethnobotanical studies and economic evaluation of medicinal plants in Taounate province (Northern Morocco). J Ethnopharmacol 86:149–158

  19. Ez zoubi Y, Bousta D, El Mansouri L, Boukhira S, Lebtar S, Sanae A, Abdellah F (2016) Phytochemical screening, anti-inflammatory activity and acute toxicity of hydro-ethanolic, flavonoid, tannin and mucilage extracts of Lavandula stoechas L. from Morocco. Int J Pharmacogn Phytochem Res 8:31–37

  20. Ez zoubi Y, Bousta D, Farah A (2020) A phytopharmacological review of a Mediterranean plant: Lavandula stoechas L. Clin Phytosci 6:9

  21. Ghasemi Pirbalouti A, Hashemi M, Ghahfarokhi FT (2013) Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Ind Crops Prod 48:43–48

  22. Gilani AH, Aziz N, Khan MA, Shaheen F, Jabeen Q, Siddiqui BS, Herzig JW (2000) Ethnopharmacological evaluation of the anticonvulsant, sedative and antispasmodic activities of Lavandula stoechas L. J Ethnopharmacol 71:161–167

  23. Gülçin Ì, Güngör Şat İ, Beydemir Ş, Elmastaş M, İrfan Küfrevioǧlu Ö (2004) Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chem 87:393–400

  24. Hong H, Landauer MR, Foriska MA, Ledney GD (2006) Antibacterial activity of the soy isoflavone genistein. J Basic Microbiol 46:329–335

  25. Karabagias IK, Karabagias VK, Riganakos KA (2019) Physico-chemical parameters, phenolic profile, in vitro antioxidant activity and volatile compounds of ladastacho (Lavandula stoechas) from the region of Saidona. Antioxidants 8

  26. Kulabas SS, Ipek H, Tufekci AR, Arslan S, Demirtas I, Ekren R, Sezerman U, Tumer TB (2018) Ameliorative potential of Lavandula stoechas in metabolic syndrome via multitarget interactions. J Ethnopharmacol 223:88–98

  27. Leber AL (2016) Agar Dilution MIC Test. Clinical Microbiology Procedures Handbook. Wiley, New York, pp 5.4.1.1–5.4.2.12

  28. Lee Y, Howard LR, Villalón B (1995) Flavonoids and antioxidant activity of fresh pepper (Capsicum annuum) cultivars. J Food Sci 60:473–476

  29. Lima MC, Paiva de Sousa C, Fernandez-Prada C, Harel J, Dubreuil JD, de Souza EL (2019) A review of the current evidence of fruit phenolic compounds as potential antimicrobials against pathogenic bacteria. Microb Pathog 130:259–270

  30. McClure JW (1975) Physiology and functions of flavonoids. In: Harborne JB, Mabry TJ, Mabry H (eds) The flavonoids. Springer, Boston, pp 970–1055

  31. Mitani T, Ota K, Inaba N, Kishida K, Koyama HA (2018) Antimicrobial activity of the phenolic compounds of prunus mume against enterobacteria. Biol Pharm Bull 41:208–212

  32. Moghaddam M, Mehdizadeh L (2015) Variability of total phenolic, flavonoid and rosmarinic acid content among Iranian basil accessions. LWT Food Sci Technol 63:535–540

  33. Mustafa SB, Akram M, Muhammad Asif H, Qayyum I, Hashmi AM, Munir N, Khan FS, Riaz M, Ahmad S (2019) Antihyperglycemic activity of hydroalcoholic extracts of selective medicinal plants Curcuma longa, Lavandula stoechas, Aegle marmelos, and Glycyrrhiza glabra and their polyherbal preparation in alloxan-induced diabetic mice. Dose-Response 17

  34. Muthuraman A, Sood S, Singla SK (2011) The antiinflammatory potential of phenolic compounds from Emblica officinalis L. in rat. Inflammopharmacology 19:327–334

  35. Nieto G (2017) Biological activities of three essential oils of the lamiaceae family. Medicines 4:63

  36. Rolain JM, Canton R, Cornaglia G (2012) Emergence of antibiotic resistance: need for a new paradigm. Clin Microbiol Infect 18:615–616

  37. Sarikurkcu C, Hanine H, Sarikurkcu RB, Sarikurkcu RT, Amarowicz R (2020) Micromeria myrtifolia: the influence of the extracting solvents on phenolic composition and biological activity. Ind Crops Prod 145:111923

  38. Silva JC, Rodrigues S, Feás X, Estevinho LM (2012) Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food Chem Toxicol 50:1790–1795

  39. Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in enzymology. Oxidants and antioxidants Part A, p 152–178

  40. Tocci N, Weil T, Perenzoni D, Narduzzi L, Madriñán S, Crockett S, Nürk NM, Cavalieri D, Mattivi F (2018) Phenolic profile, chemical relationship and antifungal activity of Andean Hypericum species. Ind Crops Prod 112:32–37

  41. Upson TM, Grayer R, Greenham JR, Williams C, Al-Ghamdi F, Chen F-H (2000) Leaf flavonoids as systematic characters in the genera Lavandula and Sabaudia. Biochem Syst Ecol 28:991–1007

  42. Verdrengh M, Collins LV, Bergin P, Tarkowski A (2004) Phytoestrogen genistein as an anti-staphylococcal agent. Microbes Infect 6:86–92

  43. Vu TT, Kim H, Tran VK, Vu HD, Hoang TX, Han JW, Choi YH, Jang KS, Choi GJ, Kim J-C (2017) Antibacterial activity of tannins isolated from Sapium baccatum extract and use for control of tomato bacterial wilt. PLoS ONE 12:e0181499

  44. Wang Q, Wang H, Xie M (2010) Antibacterial mechanism of soybean isoflavone on Staphylococcus aureus. Arch Microbiol 192:893–898

  45. WHO (2019) Antibacterial agents in clinical development: an analysis of the antibacterial clinical development pipeline. ISBN 978-92-4-000019-3

  46. Yadikar N, Bobakulov K, Li G, Aisa HA (2018) Seven new phenolic compounds from Lavandula angustifolia. Phytochem Lett 23:149–154


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Acknowledgements :




Author Information:



Yassine Ez zoubi
Biotechnology, Environmental Technology and Valorization of Bio-Resources Team, Department of Biology, Faculty of Science and Technology Al-Hoceima, Ajdir 32003, Abdelmalek Essaadi University, Tetouan, Morocco
y.ezzoubi@uae.ac.ma




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