VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 2, June 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Page Visits: 39

Doi: 10.1007/s42535-020-00115-8
Doi Link: https://doi.org/10.1007/s42535-020-00115-8
First Page: 352
Last Page: 359
Published: 07 April, 2020

Characterization of biocontrol microorganisms from the rhizoplane of Decalepis arayalpathra and screening of secondary metabolites


Abstract:

Microbial communities present in the rhizoplane of plants play an essential role in both plant growth and physiological functioning. These microbes are attracted to the volatile compounds released by plants as a by product of their metabolism. Present investigation was designed to isolate and identify microbes from the rhizoplane regions of the tissue culture raised medicinal plant, Decalepis arayapathra KMA 05 clones. Two fungal species, i.e., Cladosporium sp. F42 (FJ755822) and Epicoccum nigrum (KR094452) were isolated from the rhizoplane. In antagonistic activity assay, E. nigrum was found to inhibit the growth of Cladosporium sp., thus displaying its biocontrol potential. The GC–MS analysis of fungal extracts showed bioactive molecules that can act as anti-microbial, anti-cancerous, and plant growth-promoting molecules. Moreover, exclusive and high concentration of PPDHMP from the E. nigrum supports for its involvement in Cladosporium growth inhibition. Thus the identified microbes can be exploited as biocontrol agents and plant growth-promoting microbes for D. arayalpathra.

Vegetos

Keywords:


Rhizoplane, Microflora, n Decalepis arayalpathran , Biocontrol, Antimicrobial


References:


  1. Ahmad Z, Shahzad A, Sharma S et al (2018) Ex vitro rescue, physiochemical evaluation, secondary metabolite production and assessment of genetic stability using DNA based molecular markers in regenerated plants of Decalepis salicifolia (Bedd. ex Hook.f.) Venter. Plant Cell Tissue Organ Culture 132:497–510

  2. Baute MA, Deffieux G, Baute R, Neveu A (1978) New antibiotics from the fungus Epicoccum nigrum. J Antibiotics 31(11):1099–1101

  3. Brown AE, Finlay R, Ward JS (1987) Antifungal compounds produced by Epicoccum purpurascens against soil-borne plant pathogenic fungi. Soil Biol Biochem 19(6):657–664

  4. Butt TM, Jackson C, Magan N (eds) (2001) Fungi as biocontrol agents: progress problems and potential. Centre for Agriculture and Biosciences International, Wallingford, p 416

  5. Gamalero E, Glick BR (2015) Bacterial modulation of plant ethylene levels. Plant Physiol 169(1):13–22

  6. Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H, Rai M (2015) Endophytic actinobacteria of medicinal plants: diversity and bioactivity. Antonie Van Leeuwenhoek 108(2):267–289

  7. Harwoko H and Daletos G et al (2019) Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum. Nat Prod Res 1–9

  8. Jeyaseelan EC, Tharmila S, Niranjan K (2012) Antagonistic activity of Trichoderma spp. and Bacillus spp. against Pythium aphanidermatum isolated from tomato damping off. Arch Appl Sci Res 4(4):1623–1627

  9. Mendes R, Garbeva P, Raaijmakers JM (2013) The rhizosphere microbiome: significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms. FEMS Microbio Rev 37(5):634–663

  10. Moricca S, Ragazzi A, Mitchelson KR, Assante G (2001) Antagonism of the twoneedle pine stem rust fungi Cronartium flaccidum and Peridermium pini by Cladosporium tenuissimum in vitro and in planta. Phytopathology 91:457–468

  11. Ogorek R, Plaskowska E (2010) Epicoccum nigrum for biocontrol agents in vitro of plant fungal pathogens. Commun Agric Appl Biol Sci 76(4):691–697

  12. Park MS, Gao C, Stern HA (2011) Estimating binding affinities by docking/scoring methods using variable protonation states. Proteins Struct Funct Genet 79(1):304–314

  13. Paul D, Park KS (2013) Identification of volatiles produced by Cladosporium cladosporioides CL-1, a fungal biocontrol agent that promotes plant growth. Sensors 13(10):13969–13977

  14. Pieterse CM, Zamioudis C, Berendsen RL, Weller DM, Van Wees SC, Bakker PA (2014) Induced systemic resistance by beneficial microbes. Ann Rev Phytopathol 52:347–375

  15. Ravikumar K, Ved DK, Vijaya SR, Udayan PS (2000) 100 red listed medicinal plants of conservation concern in Southern India. Foundation for Revitalisation of Local Health Traditions (FRLHT), Bangalore, pp 261–263

  16. Shine VJ, Shyamal S, Latha PG, Rajasekharan S (2007) Gastric antisecretory and antiulcer activities of Decalepis arayalpathra. Pharm Biol 45:210–216

  17. Shweta S, Bindu JH et al (2013) Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecine from Miquelia dentata Bedd. (Icacinaceae). Phytomedicine 20(10):913–917

  18. Srivastava S, Laisram N, Ram H, Singh VP (2017) Bio-priming of tissue culture raised Decalepis arayalpathra (J. Joseph & V. Chandras.) Venter, KMA 05 clones with the phyllospheric bacterium, Methylobacterium sp. VP103. Vegetos 30:40–51

  19. Ueda H, Kurose D, Kugimiya S et al (2018) Disease severity enhancement by an esterase from non-phytopathogenic yeast Pseudozyma antarctica and its potential as adjuvant for biocontrol agents. Sci Rep 8(1):16455

  20. Vandenkoornhuyse P, Quaiser A, Duhamel M, Le Van A, Dufresne A (2015) The importance of the microbiome of the plant holobiont. New Phytol 206(4):1196–1206

  21. Vigneshwari A, Rakk D, Németh A et al (2019) Host metabolite producing endophytic fungi isolated from Hypericum perforatum. PLoS ONE 14(5):e0217060

  22. Vishwakarma K, Sharma S, Kumar V, Upadhyay N, Kumar N, Mishra R, Tripathi DK (2017) Current scenario of root exudate-mediated plant-microbe interaction and promotion of plant growth. In: Probiotics in agroecosystem. Springer, Singapore, pp 349–369

  23. Yadav RKP, Karamanoli K, Vokou D (2010) Estimating bacterial population on the phyllosphere by serial and leaf imprint methods. Ecol Soc 17(4):47–52

  24. Yim W, Woo S, Kim K, Sa T (2012) Regulation of ethylene emission in tomato (Lycopersicon esculentum Mill.) and red pepper (Capsicum annuum L.) inoculated with ACC deaminase producing Methylobacterium spp. Korean J Soil Sci Fertil 45(1):37–42

  25. Zeriouh H, de Vicente A, Perez-García A, Romero D (2014) Surfactin triggers biofilm formation of Bacillus subtilis in melon phylloplane and contributes to the biocontrol activity. Environ Microbiol 16(7):2196–2211

  26. Zhang B, Bai Z et al (2010) Microbial diversity within the phyllosphere of different vegetable species. Curr Res Technol Educ Top Appl Microbiol Microb Biotechnol 16(2):1067–1077

  27.  


  28.  


Acknowledgements :



The authors are grateful to Prof. K. S. Rao (HOD), Department of Botany, University of Delhi, for providing necessary facilities to carry out this work. The authors are thankful to the Director, CSIR-CIMAP, Lucknow, for providing the in-vitro samples to carry out this work. Financial supports from Non-Net fellowship (no. Sch/Non-NET/139/2014-15/51) from DU/UGC are gratefully acknowledged. The author is highly thankful to Dr. Balaram.


Author Information:



Shikha Srivastava
Department of Botany, Faculty of Science, University Enclave, New Delhi, India
shikhaminty@gmail.com




Pdf Download