VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 1, March 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 175

Doi: 10.1007/s42535-019-00074-9
Doi Link: https://doi.org/10.1007/s42535-019-00074-9
First Page: 11
Last Page: 20
Published: 07 November, 2019

Regeneration response of carnation cultivars in response of silver nanoparticles under in vitro conditions


Abstract:

The purpose of this study was to analyze the effect of chemically synthesized silver nanoparticles (AgNPs) on in vitro regeneration of carnation cultivars cv. Noblessa, cv. Antigua and cv. Mariposa. Number of shoots/explant of cv. Noblesse and cv. Antigua significantly increased at 6 mg/L AgNPs (average 7.33 shoots per explant) when supplemented in MS media. While cv. Mariposa showed highest regeneration rate at 8 mg/L (average 10 shoots per explant). High concentration of AgNPs (12 mg/L) in the medium enhanced rooting response, number of roots/plant, and root length as compared with control. The fresh and dry weight of regenerated plants significantly (P < 0.05) increased at 6 mg/L. DPPH based free radical scavenging activity, total antioxidant activity and reducing power potential of regenerated plants varied depending upon concentration of AgNPs in the media. To find non-enzymatic antioxidants to combat oxidative damage, total phenolics and flavonoids were also determined in the regenerated plants. The study concludes that metallic nanoparticles has significant effect on in vitro growth of carnation cultivars however concentration dependent. Furthermore, nanoparticles can be effectively used for increased in vitro shoot multiplication and regeneration of floriculture plants.

Vegetos

Keywords:


Carnation, Silver nanoparticles, In vitro, Antioxidant, Phenolic, Flavonoid


References:


  1. Abdel-Aziz MS, Shaheen MS, El-Nekeety AA, Abdel-Wahhab MA (2014) Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J Saudi Chem Soc 18:356–363

  2.  

  3. Ames BN, Shigenaga MK, Hagen TM (1995) Mitochondrial decay in aging. Biochim Biophys Acta 1271:165–170

  4. An J, Zhang M, Wang S, Tang J (2008) Physical, chemical and microbiological changes in stored green asparagus spears as affected by coating of silver nanoparticles-PVP. LWT Food Sci Technol 41:1100–1107

  5. Auffan M, Rose J, Bottero J-Y, Lowry GV, Jolivet J-P, Wiesner MR (2009) Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nature Nanotechnol 4:634–641

  6.  

  7. Bell PF, Chaney RL, Angle JS (1991) Free metal activity and total metal concentrations as indices of micro nutrient availability to barley [(Hordeum vulgare L. Klages]. Plant Soil 130:51–62

  8. Bernard F, Moghadam NN, Mirzajani F (2015) The effect of colloidal silver nanoparticles on the level of lignification and hyperhydricity syndrome in Thymus daenensis vitro shoots: a possible involvement of bonded polyamines. Vitro Cell Dev Biol Plant 51:546–553

  9. Chang S-T, Wu J-H, Wang S-Y, Kang P-L, Yang N-S, Shyur L-F (2001) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 49:3420–3424

  10. Choi O, Deng KK, Kim N-J, Ross L, Surampalli RY, Hu Z (2008) The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res 42:3066–3074

  11.  

  12.  

  13. Deb MS, Jamir N, Deb CR (2014) In vitro culture of immature embryos of Cinnamomum tamala Nees.—The role of different factors. Indian J Exp Biol 52(10):1003–1010

  14. Dipankar C, Murugan S (2012) The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids Surf B Biointerfaces 98:112–119

  15. Ghosh A, Hossain MM, Sharma M (2014) Mass propagation of Cymbidium giganteum Wall. ex Lindl. using in vitro seedlings. Indian J Exp Biol 52(9):905–911

  16.  

  17. Hemalatha S, Lalitha P, Arulpriya P (2010) Antioxidant activities of the extracts of the aerial roots of Pothosaurea. Linden ex Andre. Der Pharma Chemica 2:84–89

  18. Hernández I, Alegre L, Van Breusegem F, Munné-Bosch S (2009) How relevant are flavonoids as antioxidants in plants? Trends Plant Sci 14:125–132

  19. Javed R, Usman M, Tabassum S, Zia M (2016) Effect of capping agents: structural, optical and biological properties of ZnO nanoparticles. Appl Surf Sci 386:319–326

  20. Kähkönen MP, Hopia AI, Vuorela HJ, Rauha J-P, Pihlaja K, Kujala TS, Heinonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 47:3954–3962

  21. Kanner J, Frankel E, Granit R, German B, Kinsella JE (1994) Natural antioxidants in grapes and wines. J Agric Food Chem 42:64–69

  22.  

  23. Koontz HV, Berle KL (1980) Silver uptake, distribution, and effect on calcium, phosphorus, and sulfur uptake. Plant Physiol 65:336–339

  24. Kumar V, Ramakrishna A, Ravishankar G (2007) Influence of different ethylene inhibitors on somatic embryogenesis and secondary embryogenesis from Coffea canephora P ex Fr. In Vitro Cell Dev Biol Plant 43:602–607

  25. Kumari M, Mukherjee A, Chandrasekaran N (2009) Genotoxicity of silver nanoparticles in Allium cepa. Sci Total Environ 407:5243–5246

  26.  

  27. Lee W-M, Kwak JI, An Y-J (2012) Effect of silver nanoparticles in crop plants Phaseolus radiatus and Sorghum bicolor: media effect on phytotoxicity. Chemosphere 86:491–499

  28. Lü P, Cao J, He S, Liu J, Li H, Cheng G, Ding Y, Joyce DC (2010) Nano-silver pulse treatments improve water relations of cut rose cv. Movie Star flowers. Postharvest Biol Technol 57:196–202

  29. Ma X, Geiser-Lee J, Deng Y, Kolmakov A (2010) Interactions between engineered nanoparticles ENPs and plants: phytotoxicity, uptake and accumulation. Sci Total Environ 408(16):3053–3061

  30. Mahendra S, Zhu H, Colvin VL, Alvarez PJ (2008) Quantum dot weathering results in microbial toxicity. Environ Sci Technol 42:9424–9430

  31. Mangal M, Sharma D, Sharma M (2014) In vitro regeneration in olive. Olea europaea L. cv, ‘Frontio’from nodal segments. Indian J Exp Biol 52(9):912–916

  32. Mather C (2008) Value chains and tropical products in a changing global trade regime. International Centre for Trade and Sustainable Development, ICTSD, Geneva

  33. Mazumdar H, Ahmed G (2011) Synthesis of silver nanoparticles and its adverse effect on seed germinations in Oryza sativa, Vigna radiate and Brassica campestris. Int J Advbiotechnol Res 2:404–413

  34.  

  35. McDonald S, Prenzler PD, Antolovich M, Robards K (2001) Phenolic content and antioxidant activity of olive extracts. Food Chem 73:73–84

  36.  

  37.  

  38. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plantarum 15:473–497

  39. Neumann PM (2008) Coping mechanisms for crop plants in drought-prone environments. Ann Bot 101:901–907

  40. Oktay M, Gülçin İ, Küfrevioğlu Öİ (2003) Determination of in vitro antioxidant activity of fennel. Foeniculum vulgare. seed extracts. LWT Food Sci Technol 36:263–271

  41.  

  42. Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720

  43. Pant M (2016) A minimal cost micropropagation protocol for Dianthus caryophyllus L.–a commercially significant venture. Indian J Exp Biol 54:203–211

  44. Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341

  45. Razzaq A, Ammara R, Jhanzab H, Mahmood T, Hafeez A, Hussain S (2015) A novel nanomaterial to enhance growth and yield of wheat. J Nanosci Technol 2(1):55–58

  46. Rezaei F, Moaveni P, Mozafari H (2015) Effect of different concentrations and time of nano TiO2 spraying on quantitative and qualitative yield of soybean. Glycine max L. at Shahr-e-Qods, Iran. Biol Forum 7(1):957 (Research Trend)


  47.  


Acknowledgements :




Author Information:



Muhammad Zia
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
m.zia@qau.edu.pk
Kamran Yaqoob
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan


Abdul Mannan
Department of Pharmacy, COMSATS University, Abbotabad, Pakistan

Sobia Nisa
Department of Microbiology, University of Haripur, Haripur, Pakistan

Ghulam Raza
Division of Plant Biotechnology, NIBGE, Faisalabad, Pakistan

Riaz ur Rehman
Department of Floriculture and Horticulture, Government of Punjab, Lahore, Pakistan




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