VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles

A SOCIETY FOR PLANT RESEARCH PUBLICATION


Volume: 33, Issue: 1, March 2020


Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Views: 116

Doi: 10.1007/s42535-019-00079-4
Doi Link: https://doi.org/10.1007/s42535-019-00079-4
First Page: 31
Last Page: 39
Published: 13 November, 2019

Media optimization using Box Behnken design for enhanced production of biomass, beta-carotene and lipid from Dunaliella salina


Abstract:

Dunaliella salina a halotolerant microalga is well known for a high fatty acid and beta-carotene content, which makes it a potent source at a commercial level. The current study focuses on optimizing commercially known media using Box Behnken design to attain higher yields of biomass, beta-carotene, and lipids simultaneously. The optimal medium conditions as per response surface methodology were glucose, potassium nitrate, sodium chloride at a concentration of 13.23 g/L, 3.145 g/L and 35.6 g/L, respectively while maintaining the concentration of other nutrients unchanged. Maximum yield of biomass, beta-carotene and lipid productivity attained experimentally using the optimized media was 1.24 g/L, 6.07 mg/g and 20.7 mg/L/day, respectively than their original values i.e., 0.571 g/L of biomass, 4.18 mg/g of beta-carotene and 13.2 mg/L/day of lipid content. Biomass yield was increased by 2.17 folds, beta-carotene and lipid were increased by 1.45 folds and 1.56 folds, respectively.

Vegetos

Keywords:


Beta-carotene, Lipids, Box Behnken design, Response surface methodology


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Acknowledgements :



This study was supported by Department of Biotechnology, Delhi Technological University, New Delhi.


Author Information:



Shruti Ahuja
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

Arpita Roy
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India


Lakhan Kumar
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

Navneeta Bharadvaja
Plant Biotechnology Laboratory, Department of Biotechnology, Delhi Technological University, New Delhi, India

navneetab@dce.ac.in




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