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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :
Year :2008, Volume : 21, Issue :2
First page : (125) Last page : (128)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

In-vitro regeneration of Tinospora cordifolia Miers; a medicinal climbing shrub

Afshan Tabassum, Nag KK

Department of Botany, Ranchi University, Ranchi-834 008, Jharkhand.

The nodal explants of Tinospora cordifolia, after surface sterilization with 70% ethanol for 1 minute and 0.1% mercuric chloride for 5 minutes, have been cultured on nutrient medium containing Murashige and Skoog's salts, vitamins, surcrose and various concentrations and combinations of BAP and NAA. The nodal segments exhibit basal callusing after 2 weeks and emergence of shoots from the axillary bud after 3 weeks. The emerging shoots have been sub-cultured to a fresh medium with NAA (1 ppm) and BAP (2 ppm). After 2 weeks of first sub-culture, shoots emerged from the single shoot along with root. The sub-culture of the callus on medium containing BAP (2 ppm) and NAA (1 ppm) has resulted only in shoot differentiation.




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