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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :
Year :2012, Volume : 25, Issue :1
First page : (163) Last page : (170)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Inter-Varietal Variations to Phenolic Compounds in Okra (Abelmoschus esculentus) Germplasm

Vaishali*, Agarwal Rachna, Kumar Rajendra, Singh Bijendra1

Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, 250 110 India
1Department of Horticulture, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, 250 110 India

* Corresponding author email: vaishali_a21@yahoo.com

The quantitative analysis of total phenolics extracted from the leaves of 22 varieties of Okra (Abelmoschus esculentus) indicated that the amount of total phenolics were relatively low in susceptible varieties than the resistant and hybrid varieties. The qualitative analysis of total phenolics using TLC indicated the tentative presence of ortho and para coumaric acids in some varieties of Okra. To know the identity of the compounds, okra samples were analysed by HPLC and were compared with the standards like caffeic acid, o-coumaric acid, sinapic acid, p-coumaric acid and ferulic acid. The chromatograms showed that the peak number with significant area percent varied from 3 to 8. It was concluded that the resistant varieties have all the four compound at peak no 1–4 whereas the hybrid varieties lack the compound at peak no 4. The susceptible varieties also showed difference of pattern but none of them showed the presence of all four compounds. Thus all the four compound are necessary to provide resistance to the okra germplasm.




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