Diversity Analysis of Tomato Genotypes Using RAPD Markers and High-Performance Liquid Chromatography In Relation To β-Carotene Content
Salunke D S, Jadhav A S, Pawar B D*, Kale A A, Chimote V P
State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, Rahuri, 413722, India
*Corresponding author Email: firstname.lastname@example.org
This study was conducted to investigate the β-carotene content and genetic diversity of 18 red fruited and 12 orange fruited tomato genotypes, using high-performance liquid chromatographic and RAPD markers respectively. β-carotene content was significantly higher in orange fruited tomato genotypes as compared to red fruited tomato genotypes. The highest β-carotene content was found in orange fruited genotype 9A-1-1 (43.7) μg/g) whereas lowest β-carotene content was observed in Pusa Ruby (6.6) μg/g). Genetic diversity in thirty tomato genotypes was analyzed by RAPD markers generated by 23 random primers. The amplification profile consisted of 202 fragments of size ranging from 174 bp to 3650 bp of which 39 were monomorphic and 163 were polymorphic with 80.69% polymorphism. The number of bands generated by each primer varied from 4 (OPM-18) to 13 (OPK-04) with an average of 8.78 fragments per primer. The percentage ofpolymorphic bands with different primers ranged from 40 to 100%. The efficiency of RAPD marker for cultivar identification was found 2.97% as only six fragments are cultivar specific. Orange fruited genotype NBC showed three unique fragments, OPK 03–968 bp, OPL 17–322 bp and OPM 12–2349 bp. The similarity coefficients detected by RAPD ranged from 0.63 to 0.96 which revealed existence of limited genetic variation among tomato genotypes. The consensus tree constructed showed three major clusters. First cluster comprise of 16 genotypes, second cluster of 13 genotypes and the red fruited tomato genotype M-1-2B formed third independent cluster. The RAPD technology proved useful in describing genetic diversity among tomato genotypes.