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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :
Year :2012, Volume : 25, Issue :2
First page : (127) Last page : (135)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Molecular Detection And Characterization of Phytoplasma Associated with Periwinkle

Kumar Sanjeev*, Byadgi A. S.

Department of Plant Pathology, College of Agriculture University of Agricultural Sciences, Dharwad-580 005, Karnataka

*Corresponding author Email: sk71119@gmail.com

Phytoplasmas are cell wall-less, non-helical prokaryotes that colonize plant phloem and insects gut wall and are known to cause devastating losses in crops and natural ecosystems worldwide. Amplification and band of ∼1.8 kb and ∼ 1.25 kb was obtained with little leaf disease of periwinkle and parthenium phyllody samples, but not other phytoplasma suspected samples with the phytoplasma specific primer pairs P1/P7 and R16F2/R2 in first and second round PCR respectively confirming its phytoplasmal etiology. The partial sequence (GenBank accession: HQ127073) of 16S rRNA gene of the present isolate shared 99 per cent homology with reported Sugarcane yellow leaf phytoplasma isolate from Brazil belonging Candidatus phytoplasma asteris (Group 16Sr I-B) isolate. Evolutionarily closeness to subgroup 16SrI-B (‘Candidatus Phytoplasma asteris’) and virtual RFLP Pattern similarity to 16SrI-B predict that the little leaf disease of periwinkle from Dharwad belongs to 16S rDNA I-B phytoplasma group.




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