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Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/j.2229-4473.27.1.027
Year :2014, Volume : 27, Issue :1
First page : (174) Last page : (180)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


Characterization of 27 molecular markers linked to Barley Yellow Dwarf Virus (BYDV) resistance genes in Elymus shandongensis (Triticeae, Poaceae)

Liu Quanlan, Zhang Rui, Yan Xue-Bing1,*

Department of Bioengineering and Biotechnology, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao-266042, China
1College of Animal and Veterinary Science, Henan Agricultural University, 450002, Zhengzhou, China

*Corresponding author E-mail:

Barley Yellow Dwarf Virus (BYDV) is a pathogen caused serious yield losses in cereals, which may become even more important due to its intrinsic adaptation and global warming. As a perennial species in Triticeae, Elymus shandongensis contains genes resistant to BYDV. The 27 molecular markers linked to BYDV resistance genes developed from Triticeae species were characterized. The 25 loci were polymorphic, and 2 loci were monomorphic in this study. The number of alleles ranged from 1 to 6. The effective number of alleles (Ne) of each primer ranged from 1 to 1.932 (mean= 1.624). The Shannon's information index (I) of each primer ranged from 0 to 0.662 (mean= 0.499), and the unbiased genetic diversity (He) ranged from 0 to 0.481 (mean= 0.353). The population from the Lingshan island (PL) showed a lower level of genetic variation (P =80.25%, I =0.477, and He =0.338), whereas the population from the surrounding areas of the Laoshan reservoir (PS) had a higher level of genetic diversity (P =90.12%, I =0.520, and He =0.368). Four private alleles were found in PL and 1 private allele was found in PS. The results indicated the 27 markers could be used in future genetic study of E. shandongensis that might be useful in a context of wheat breeding, germplasms conservation, finding useful resistant genotype to BYDV and adaptive evolution at resistance loci to virus in natural populations.