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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2015.00014.2
Year :2015, Volume : 28, Issue :1
First page : (109) Last page : (112)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Authentication of Hybrid Purity in Eggplant (Solanum melongena L.) Through RAPD Analysis

Shukla Rajshree*, Mehta Nandan, Saxena Ritu R, Verulkar S B1

Department of Genetics & Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya, Raipur- 492 012 Chhattisgarh, India
1Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur- 492 012, Chhattisgarh, India

*Corresponding author E-mail: shuklarajshree06@gmail.com

For testing seed purity and trueness, GOT (Grow Out Test) is a long term process and therefore, present study was carried out during Rabi-2011. The experimental materials used in the study comprised of four parents viz., Green Long (GL), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Punjab Sadabahar (PS) and their six F1’s and standard check PH-6. RAPD marker was used to test the hybridity. Out of twenty-nine, only four primers showed polymorphism. namely OPA -14, OPA-08, OPH-01 and OPH-20. According to banding pattern for the primer OPH-01, hybrid between P4 and P2 (IBWL-2007-1 X Green Long) resembles to male parent i.e., Green Long (P2) and in same reciprocal cross P2 and P4 (Green Long X IBWL-2007-1), the banding patterns of F1 hybrid resembles male parent i.e. IBWL-2007-1 (P4). In another hybrid, between P3 and P4 (Punjab Sadabahar X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e., IBWL-20071 (P4). For the primer OPH-20, F1 hybrid between P1 and P3 (Punjab Sadabahar X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e. IBWL-2007-1 (P3).For the primer OPA14, F1 hybrid between P3 and P4 (IBWL-2007-1 X Pusa Purple Long) banding pattern of F1 hybrid resembles to male parent i.e. Pusa Purple Long (P4) and in the same reciprocal cross P4 and P3 (Pusa Purple Long X IBWL-2007-1) banding pattern of F1 hybrid resembles to male parent i.e. IBWL-2007-1 (P3). For primer OPA-18, in F1 hybrid between P1 and P3 (Punjab Sadabahar X IBWL2007-1), the banding pattern resembles to male parent i.e. IBWL-2007-1 (P3).




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