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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2015.00044.0
Year :2015, Volume : 28, Issue :2
First page : (103) Last page : (110)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Comparative Quantitative Gene Expression analysis Between Salinity Tolerant and Susceptible Varieties of Chickpea

Mittal Neha1,2, Saxena R S2, Yadav Renu1,4, Bhardwaj Juhi1, Yadav Neelam8, Kumar Ashwani1,3, Yadav Hemant6, Kumar Rajendra1,5,7,*

1Department of Biotechnology, S.V.P. University of Agriculture & Technology, Meerut-250110, (UP), India
2Department of Botany, Meerut College, C.C.S. University, Uttar Pradesh, India
3Department of Botany, Ch. Charan Singh (PG) College, Heonra (Saifai), Etawah, (UP), India
4Directorate of Science & Innovation, Ami-ty University, Noida
5Division of Genetics, Indian Agricultural Research Institute, New Delhi-110012
6Di-vision of Genetics & Molecular Biology, National Botanical research Institute, Lucknow-226001
7U.P. Council of Agricultural Research, Lucknow, India-226010
8Center For Food Technology, Allahabad University, Allahabad

*Corresponding author's E-mail: rajendrak64@yahoo.co.in

Salinity is one of the greatest limiting factors in agriculture. Therefore, the ability of plants to adapt saline conditions is crucial to sus-tain worldwide crop production. Present study is meant to identify gene specific markers for salt tolerance in Chickpea. Selected chickpea geno-types were already characterized for salt tolerance and susceptibility. Sixteen gene specif-ic primers were designed from already identified abiotic stress resistant genes from database among which five were used for semi-quantitative reverse transcription polymerase chain reaction. The expression levels of two of them allow discrimination between toler-ant and susceptible genotypes. In tolerant genotypes (JG-62 and Pusa 1053) the expression level of these genes was higher as compared to susceptible genotypes (BG-256 and ICCV2). For primer 13 the relative fold increase in gene expression was 10 times as compared to 2 times in susceptible plants. For primer 6 the relative fold increase in gene expression was 5 folds in Pusa 1053 (tolerant) as compared to 4 folds in other geno-types. Gene expression was observed to be maxi-mum at 200mM concentration in almost all gen-otypes for above two gene specific primers. So, primer 13 is a potent gene for gene pyramiding in susceptible varieties for salt stress.




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