Effect of a Potent Cytokinin Thidiazuron (TDH) on the Regener-ation Potential of Leaf and Petiole Explants of Cauliflower (Brassica oleracea L var. botrytis)
Gaur Ayesh*, Kumar Pankaj, Srivastava D. K.
Department of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan-173230, Himachal Pradesh, India
*Corresponding author E-mail: firstname.lastname@example.org, email@example.com
Plant regeneration studies were carried out using leaf and petiole explants on forty dif-ferent combinations and concentrations of TDZ i. e. TDZ, TDZ+IAA, TDZ+NAA and TDZ+Adenine. Among the forty combinations tried, the high percent shoot regeneration was observed in peti-ole explants (76%) on MS medium supplemented with 0.22mg/l TDZ whereas in case of leaf ex-plants only 44% shoot regeneration was achieved on MS medium supplemented with 0.77mg/l TDZ+ 0.087 mg/l IAA. For root regener-ation the in vitro regenerated shoots were cul-tured on MS medium containing different con-centration of IAA and NAA. Hundred percent root regeneration was observed on the MS medi-um supplemented with 0.05mg/l and 0.20mg/l NAA respectively. The regenerated plantlets were transferred to the pots containing cocopeat and acclimatized. The regenerated plantlets were morphologically similar. An efficient and repro-ducible plant regeneration protocol has been standardized in cauliflower cv. Pusa Snowball K-1 and is being used for Agrobacterium- mediated cry1Aa gene transfer studies.