Indirect Organogenesis in Bittergourd (Momordica charantia L.) Using Shoot tips as Explant
Resmi J.*, Sreelathakumary I.
Department of Olericulture, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram-695522, Kerala, India
*Corresponding author E-mail: email@example.com
The induction of potentially organogenic callus and better proliferation of callus successfully from shoot tip explants of bittergourd was obtained either on the MS medium supplemented with IBA (4.00 mg l−1) + BA (0.50 mg l−1) + 2, 4-D (2.00 mg l−1) or NAA (2.00 mg l−1) + BA (0.50 mg l−1) + 2, 4-D (2.00 mg l−1) in combination with 30.00 g l−1 sucrose and 6.30 g l−1 agar level. Shoot regeneration response from shoot tip derived callus was best on MS medium fortified with 0.05% AC + 1.00 mg l−1 BA with earlier regeneration, highest regeneration percentage, lengthy adventitious shoot and more number of leaves under light conditions. Efficient rooting was achieved on MS fortified with IBA (1.00 mg l1).