Analysis of Genetic Variations in Eleusine coracana (L.) genotypes using ISSR Molecular Markers
Garg Rashmi, Gupta Shalani*, Sengar R S1, Bhatnagar S K2
Department of Molecular Biology & Genetic Engineering, College of Biotechnology, Sardar Vallabhbhai Pa-tel University of Agriculture & Technology, Meerut-250 110, U.P.
1Department of Agriculture Biotechnology, S V P University of Agriculture & Technology, Meerut-250 110, U.P.
2Department of Cell Biotechnology, College of Biotechnology, S V P University of Agriculture & Technology, Meerut-250 110, U.P.
*Corresponding author E-mail: firstname.lastname@example.org
Inter Simple Sequence Repeat (ISSR) markers were used to assess the genetic rela-tionships among 27 finger millet genotypes. Ten 5 ’anchored and ten 3 ’anchored ISSR primers were initially screened for PCR amplifications. From a total of 82 DNA fragments produced, 76 (92.68%) were found to be polymorphic. The number of bands produced by different markers was ranging from 2 to 8. PIC value and Rp value were ranging from 0.143 to 0.983 and 0.52 to 11.56, respectively. The genetic similarity matrix varied from 0.43 to 0.98 in these genotypes. Dendogram analysis revealed formation of two major groups, one with five subgroups compris-ing 10 genotypes and the other one consisted of 17 genotypes in five subgroups.. The results re-vealed that ISSR markers could be efficiently used to quickly access the genetic variation available in the finger millet genotypes.