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Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2015.00094.4
Year :2015, Volume : 28, Issue :4
First page : (122) Last page : (130)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


Assessment of Genetic Diversity in Indian Mustard [Brassica juncea (l.) Czern & Coss.] for High Temperature Tolerance using SSR Markers

Nanjundan J.*, Thakur A. K., Singh K. H., Verma Vandana

Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan (321 303)-India

*Corresponding author E-mail:,

Estimation of genetic diversity had been carried out in a set of 21 germplasm accessions of Indian mustard for high temperature toler-ance trait using SSR markers. These 21 acces-sions were comprised of 11 high temperature tolerant lines, 6 susceptible and 4 checks, which were obtained after screening of 415 accessions of Indian mustard germplasm for high tempera-ture tolerance at seedling stage. Of the 106 SSR markers evaluated, 54 resulted into amplification products of different sizes, of which 19 were found polymorphic with a total of 52 alleles, at an average of 2.74 alleles per locus. The UPGMA dendrogram revealed the presence of high level of genetic diversity among the accessions evalu-ated. The similarity coefficient ranged between 0.32 and 0.90 with a mean value of 0.61. High temperature tolerant accessions identified in this study along with the information on genetic di-versity provide some useful guides for plant breeders in selecting genetically diverse parents for the crossing program to evolve cultivars pos-sessing high temperature tolerance along with high yielding potential than the existing culti-vars.