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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2015.00084.1
Year :2015, Volume : 28, Issue :4
First page : (54) Last page : (61)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Molecular Marker analysis Reveals Genetic Diversity among Wheat (Triticum aestivum L. em. Thell) Genotypes varying for Thermo-Tolerance

Goyal Meenu*, Kumar Pardeep1, Dhillon Santosh

Department of Molecular Biology and Biotechnology, CCS Haryana Agricultural University, Hisar-125 004
1ICAR-National Bureau of Plant Genetic Resources, New Delhi-110012, India

*Corresponding author E-mail: goyalmeenu333@gmail.com

High temperature stress limits wheat production worldwide. Several studies reflect that genotypic variability exists for thermo-tolerance in wheat. In this study, genotypic varia-bility for thermo-tolerance was dissected at mo-lecular level. We accessed 20 selected bread wheat genotypes (10 thermo-tolerant and 10 thermo-sensitive) for genetic diversity analysis using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. In RAPD analysis, a total of 126 alleles were detected using 18 primers, among which 76 alleles (60.3%) were polymorphic. In ISSR analy-sis, using a total of 11 ISSR primers, 95 am-plified bands were obtained of which 46 (48.4%) were polymorphic. The average num-ber of polymorphic bands was higher in case of RAPDs (4.22) as compared to that in ISSRs (4.18). The clustering dendrogram generated by ISSR based similarity matrix, segregated thermo-tolerant and thermo-sensitive genotypes better than the RAPD based clustering dendrogram. One of ISSR primers (ISSR-41) amplified a ≈2800 bp band which was able to distinguish all the thermo-tolerant and sensitive genotypes except one. This marker identified in the study may po-tentially be used in marker assisted selection to develop thermo-tolerant wheat varieties.




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