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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2015.00083.X
Year :2015, Volume : 28, Issue :4
First page : (48) Last page : (53)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Standardization of Techniques for in vitro Multiplication of Gynoecious Line in Bitter Gourd

Saha Swati*, Behera T. K.

Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi, 110012, India

*Corresponding author E-mail: swatisaha1980@gmail.com

Micro-propagation can be exploited for maintenance and mass multiplication of gynoe-cious lines in bitter gourd for hybrid seed pro-duction. Therefore, standardization of protocol for rapid in vitro multiplication will also help in development of double haploid (DH) plants in bitter gourd, which may enhance the ability to develop homozygosity in a short period particu-larly for gynoecious trait. Among the explants, the shoot tip performed better than the nodal explant in all respects. T4 treatment, which is a combination of spray of gentamycin (100 mg/l) + agitation of explants in carbendazim (0.2%) + Mancozeb (0.2%) HQC (200 mg/l) is best for the pretreatment of cultures. Among the stages, 45 days old explants are comparative better than 60 days old. Treatment with mercuric chloride (HgCl2) for 3 minutes duration recorded the maximum in vitro survival of the shoot tip of DBGy-201 (57.96%).




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