Evaluation of Abiotic and Biotic Elicitors for Induction of Defense Related Enzymes in Pea against U. Viciae-Fabae (Pers.) J. Schrot
Upadhyay Vinod1,*, Kushwaha KPS1, Pandey Puja1
1Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
*Corresponding author: Vinod Upadhyay, Regional Agricultural Research Station, Assam Agricultural University, Gossaigaon, Assam-783360, India, Tel: +91 5944 233 320, E-mail: email@example.com
Total of fifteen elicitors tested alone/or in combination for induction of defense related enzymes in pea against U. viciae-fabae (Pers.) J. Schrot results in significant induction of total phenols, peroxidase, polyphenol oxidase and phenylalanine ammonia lyase in all the treatment as compare to control. Salicylic acid, Pseudomonas fluorescens, salicylic acid + Pseudomonas fluorescens were found most effective in induction of total phenols and peroxidase at 72 hrs after spray of elicitors. Polyphenol oxidase induction was found significantly high in oxalic acid, Pseudomonas fluorescens + Trichoderma harzianum and chitosan + Pseudomonas fluorescens at 72 hrs after spray of elicitors. Among all the treatments, maximum induction of Phenylalanine ammonia lyase activity was found in oxalic acid, Trichoderma harzianum + Pseudomonas fluorescens and isonicotinic acid + Trichoderma harzianum after 48hrs of spray of elicitors. Effect of different elicitors on percent disease index (PDI) 20 days after inoculation with uredospores of U. viciae-fabae showed least PDI in salicylic acid, Trichoderma harzianum + Pseudomonas fluorescens and chitosan + Pseudomonas fluorescens treated plants.