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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2016.00085.9
Year :2016, Volume : 29, Issue :3
First page : (158) Last page : (173)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Genetic Diversity in Vegetable and Grain Type Soybean Genotypes Identified using Morphological Descriptor and EST-SSR Markers

Khanande AS1, Jadhav PV1,*, Kale PB1, Madavi SM1, Mohari MP2, Nichal SS2, Sonkamble PA3, Wandhare MR2, Walke RD4, Varghese P5, Nandanwar RS2

1Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Agricultural University, Maharashtra, India
2Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Agricultural University, Maharashtra, India
3Seed Technology Research Unit, Dr. Panjabrao Deshmukh Agricultural University, Maharashtra, India
4Department of Agricultural Economics and Statistics, Dr. Panjabrao Deshmukh Agricultural University, Maharashtra, India 5Genetics and Plant Breeding, Agharkar Research Institute, Pune, Maharashtra, India

*Corresponding author: PV Jadhav, Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Agricultural University, Maharashtra, India, Tel: +919423520822, E-mail: jpraveen26@yahoo.co.in

Thirty four soybean genotypes were evaluated for morphological, quality traits and genetic parameters. Correlation and path coefficients were studied for some traits. Analysis of variance and mean performance for various traits revealed significant differences diversity between all the genotypes for traits studied. The genetic diversity among the soybean genotypes was analyzed using ten expressed sequence tag derived simple sequence repeat (EST-SSR) markers. Atotal of 27 alleles were detected from 334 amplicons with an average of 2.7 alleles per locus. The polymorphism information content (PIC) values of EST-SSR markers ranged from 0.334 to 0.837 with an average of 0.559. These grain and vegetable soybean genotypes could be divided into 7 subgroups based on similarity matrix and arithmetic average (UPGMA) cluster with no correlation between genetic and morphological diversity. The analysis clearly indicated that even with the EST-SSR primers, reliable estimation of genetic diversity among the population could be obtained.




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