Morphological and molecular based characterization of different thermo-sensitive genetic male sterile (TGMS) lines in Rice (Oryza sativa L.)
Kumar Pardeep*, MK Nautiyal, Kumar Pankaj, Kumar Kuldeep
Department of Genetics and Plant Breeding, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India
*Corresponding author: Pardeep Kumar, Department of Genetics and Plant Breeding, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India, E-mail: email@example.com
The present investigation was carried out at the Norman E. Borlaug Crop Research Center of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand (India) during 2013 and 2014. The eighteen TGMS lines were characterized based on molecular and morphological data. Result on morphological and molecular characterization of TGMS lines showed that, not a single line was observed good for all the traits, different lines good for different traits. Among the eighteen TGMS lines ten lines viz, TGMS-2, TGMS-3, TGMS-4, TGMS-5, TGMS-7, TGMS-8, TGMS-10, TGMS-11, TGMS-14 and TGMS-15 showed complete sterility at average temperature 28.53 to 28.92°C. Based on the molecular genetic diversity data indicated that total of 47 alleles were amplified using sixteen SSR markers in the present study of eighteen TGMS lines all were found polymorphic except for one marker. The range of alleles was 2 - 5, while the average number of alleles per primer was 2.93 and thirteen rare alleles found in different lines. The polymorphic information content (PIC) for these sixteen SSR markers ranged from 0.1780 to 0.3750 with mean value 0.2543. The range of Jaccard's similarity coefficient was found to vary from 0.46 (TGMS-7 and TGMS-16) to 0.97 (TGMS-11 and TGMS-12). The UPGMA based dendogram constructed using Jaccard's similarity coefficient of SSR marker data divided eighteen lines into three clusters. Association study between the banding pattern of different markers and spikeletes fertility/sterility of TGMS lines showed that four lines out of eighteen were fertile. These fertile lines separated to other sterile lines by three markers with unique bands. TGMS-6 and TGMS-9 showed 200bp specific band by RM 324 marker, TGMS-1 and TGMS-18 showed 180bp and 200bp unique bands and in TGMS-1 also showed 180bp unique band with RM 254 marker, it indicated that the unique bands 180bp and 200bp generated by different markers in different lines responsible for fertility. This molecular diversity analysis may be useful for identification of TGMS lines or may be used for marker assisted selection (MAS) for two line hybrid development.