Regeneration potential of Ascocentrum ampullaceum (Roxb.) Schlter through inflorescence culture
Shadang R1, Dwivedi P2,*, Hegde SN1
1State Forest Research Institute, Itanagar, Arunachal Pradesh, India
2Department of Plant Physiology, Institute of Agricultural Sciences, Baranas Hindu University, Varanasi, India
*Corresponding author: Padmanabh Dwivedi, Department of Plant Physiology, Institute of Agricultural Sciences, Baranas Hindu University, Varanasi, India, Tel: 0542-6701112; E-mail: firstname.lastname@example.org
The paper deals with the in vitro regeneration response of immature inflorescence segments (buds, nodes and internodes) of Ascocentrum ampullaceum explants. The nutrient medium used played a key role in induction and multiplication of callus, protocorm like bodies and shoot. Inflorescence buds cultured in V&W medium supplemented with 15% coconut milk and 2% sucrose produced highest (80%) protocorm like bodies formation without any growth regulator. Inflorescence node culture in MKC medium supplemented with only 15% CM, and 15% CM and 1.0 mg l−1 NAA produced highest callus (70%) and plbs (50%) formation, respectively. Incorporation of 15% CM in all the media proved best for formation of callus and plbs. Our results suggest that Ascocentrum ampullaceum, which regenerates poorly in nature, can be micropropagated using inflorescence based explants.