Screening and bioactivity measurement of high altitude medicinal plants of Nepal
Sharma Deepak1,*, Shrestha Tara1, Rapal Naresh1, Poudel Satiz C1, Shrestha Sujan1, Sharma Surekshya1, Poudel Krishna P2, Lamichane Janardan1
1Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal
2Department of Chemistry, Trichandra Multiple college, Ghantaghar, Kathmandu, Nepal
*Corresponding author: Deepak Sharma, Department of Biotechnology, Kathmandu University, Dhulikhel, Kavre, Nepal, Tel: 00977-9849657056; E-mail: email@example.com
Ten different plants collected from various area of Khaptad National Park, one of the famous National Parks renowned for the source of medicinal herb located at an altitude from 1,000 m to 3,300 m, P. edgeworthii, B. petiolaris, E. strobilifera were found to have potent cytotoxicity effect while three others, T. foliolosum, B. diffusa and A. spectabilis with LC50 of 64.50, 13.59, 6.14 respectively showed very strong cytotoxicity effect against brine shrimp nauplii. The DPPH scavenging assay of all those plants was found to vary from very high to low. B. petiolaris (13.49%) showed almost the same scavenging activity to that of Ascorbic acid (17.20%) followed by E. strobilifera (6.35%) and H. salicifolia (2.38%) at a concentration of 0.5 μg/ml. The antimicrobial activity against five microorganisms (Bacillus cereus, Bacillus subtillis, Klebsiella pneumonia, Bacillus thuringiensis and Enterobacter), showed a remarkable Zone Of Inhibition (ZOI). H. salicifolia showed 6.5 mm ZOI against Bacillus cereus, 7 mm against Bacillus thuringiensis and 6 mm against Klebsiella pneumonia at 25 μg/ml whereas A. spectabilis showed 9 mm as highest ZOI against Enterobacter at 25 μg/ml.