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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2017.00133.1
Year :2017, Volume : 30, Issue :2
First page : (36) Last page : (42)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Anticonvulsant Effect of Gastrodia Rhizome Processed at Different Steaming Times with Scavenging NO and Antiinflammatory Activity

Shin Mi-Rae1, Seo Bu-Il1, Kwon OJ2,*, Roh Seong-Soo1

1Department of Herbology, College of Korean medicine, DaeguHaany University, 136, Shinchendong-ro, Suseong-gu, Deagu42158, Republic of Korea
2Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27, Sampoong-ro, Gyeongsan-city, Gyeongsangbuk-do, 38542, Republic of Korea

*Corresponding author: O Jun Kwon, Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27, Sampoong-ro, Gyeongsan-city, Gyeongsangbuk-do, 38542, Republic of Korea, Tel: 82-53-770-2098; E-mail: ojkwon@irpe.or.kr

Purpose

To evaluate Anticonvulsant effect of Gastrodia Rhizome processed at different steaming times in Pilocarpine, Pentylenetetrazol (PTZ), and Strychnine induced mice seizure model.
Methods
Gastrodia rhizome extracts divided with or without steaming process. Gastrodin content of all extracts was evaluated to use by High-Performance Liquid Chromatography (HPLC). In vitro, Gastrodia rhizome extracts were evaluated for its antiimflammatory activity by NO, DPPH and ABST assay in RAW 264.7 cells. Furthermore, we examined anticonvulsant activites in Pilocarpine, PTZ, and Strychnine induced seizure animal model.
Results

The more increase of steaming time with GR, resulted the more contents of gastrodin, total phenol and flavonoid, more effective free radical scavenging activity (DPPH and ABST assay) and significant reduction of inflammatory biomarker (NO and TNF-α). And steamed GR significantly reduced a strong seizure symptom of convulsion induced by medicine compared to unsteamed GR (p<0.01).
Conclusions
These results suggest that the steamed GR exert the efficient anticonvulsant effect via the more contents of gastrodin and antioxidant and antiinflammatory activity




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