A Simple Method to Study Host-Pathogen Interaction in Sesamum Indicum
Sharma A*, Sikarwar M, Gupta P, Srivastava N
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India
*Corresponding author: Anubhuti Sharma, Department of Bioscience and Biotechnology, Banasthali Vidyapith, P.O. Banasthali Vidyapith, Rajasthan, India, Tel: 01438-228302; E-mail: firstname.lastname@example.org
Differential activation of early defense responses like increased activity of phenylalanine ammonia-lyase (PAL), peroxidases (PO), and pathogenesis proteins (PR proteins) predisposes the host plant resistance against pathogens. Therefore, in the present study interaction between this defenses related compounds and pathogen (Macrophomina phaseolina) was used as new strategy to enhance sesamum defense responses against root rot disease. There was a marked increase in these biochemical compounds in pathogen treated sesamum plants after pathogen attack. The investigation was carried after infection, which shows increase in activities of all defense related proteins to varying degrees of infection. Interestingly, there are significant changes in cytoplasmic proteins under pathogen inoculation. In the control plants, 8 major bands and 3 minor bands are obtained. In contrast, in the inoculated plants 8 major and 4 minor bands are obtained, which however, are of different Rf-values than the control plants. The differences in the protein are more pronounced in the molecular mass range of 15 to 36 kda and 40 to 59 kda. Individual protein bands obtained through SDS-PAGE were further quantified by scion image programme. This programme gives the peak area values of the corresponding band. The peak area denotes the densities of protein bands on the gel. A comparison of the densities of the protein bands in control and inoculated plants amply demonstrated that exposure to pathogen not only results in higher protein amounts but synthesis of a few new proteins, possibly PR-proteins. The biochemical approach described in this paper should provide the basis for further efforts concentrating on genetic regulation of sesamum in the form of transcript accumulation for expression of disease resistance proteins.