Assessing Genetic Diversity of Wheat (Triticum aestivum L.) using Simple Sequence Repeats Markers
Singh Naresh Pratap*, Vaishali, Goswami Akansha1, Singh B.1
Department of Agriculture Biotechnology, S V P University of Agriculture & Technology , Meerut (U.P), India
1Department of Horticulture, S V P University of Agriculture & Technology , Meerut (U.P), India
*Corresponding author: Naresh Pratap Singh, Department of Agriculture Biotechnology, S V P University of Agriculture & Technology, Meerut (U.P), India, Email: firstname.lastname@example.org
Genetic variability of broad range of cereal crops may lead to reduction in the yield under abiotic and biotic stresses. In present study Genetic diversity of the twenty wheat (Triticum aestivum L.) varieties was analyzed at the DNA level using 19 simple sequence repeats (SSR) markers. The wheat 16 SSR markers out of 19 had detected 40 alleles with an average of 2.5 alleles per primer. The number of alleles per primer ranged from 1 to 5. The resolviong power (Rp) of each primer ranged from 1.0 for the CWM119 to 5.2 for XGWM-293-7A with an average of 2.46. The gene diversity based on SSR markers was ranged from 0.36–0.88 with an average value of 0.62. The similarity coefficient values and dendogram revealed that genotype HD 2177 and HUW 37 have lowest similarity and HD 2177 and K 9533 display the greatest genetic similarity out of 20 wheat varieties. As a result, genetically diverse parents can be identified, increasing the usefulness of variety collection by broadening the genetic base of wheat varieties and also indicates that SSR markers could characterize and discriminate between them. Presnt results suggest that the classification based on genotypic markers of these wheat genotypes will be useful for wheat breeders to plan crosses for positive traits and also indicate the positive correlations between gene diversity, number of alleles and resolving power of markers. The results obtained suggested that the wheat microsatellite primers can be used to distinguish all genotypes used and to estimate their genetic diversity.