Characterization of Cultivated and Wild Genotypes of Hot Pepper using CAPS Markers
Mangal Manisha*, Srivastava Arpita, Gosavi Gokul, Kalia Pritam
Division of vegetable Science, Indian Agricultural Research Institute-New Delhi, 110012
*Corresponding author: Manisha Mangal, Division of Vegetable Science, Indian Agricultural Research Institute-New Delhi, 110012, E-mail: firstname.lastname@example.org
Diversity of twenty four Capsicum genotypes representing five species with 19 genotypes from C. annum, two from C. fruitescens and one each from C. baccatum, C. chinense and C. chacoense has been analyzed using a set of 27 CAPS markers uniformly distributed throughout the pepper genome. These 27 CAPS loci amplified a total of 95 alleles among the 24 genotypes with 1 to 10 alleles per loci. The average number of alleles per loci was found to be 3.51. The highest polymorphism information content (PIC value) was observed to be 0.844 for marker Hba181H07SP6 located on linkage group 4. Hierarchical analysis distributed all the genotypes under study into two major clusters each including different species under study with genetic similarities in the range of 59–80% and a mean value of 70%. It was observed that genotypes were randomly distributed in each cluster and there was no clear cut pattern. Even the Chilli leaf curl resistant/tolerant lines were found to fall in both the major groups such that WBC-sel-5 and DLS-sel -10 were present in Cluster I and DKC-8, CJL-S-1, Tiwari and DCL-2 in Group II. Other cultivated Capsicum spp such as C. chinenese, C. baccatum and C. fruitiscens and wild relative C. chacoense were grouped in Cluster II.