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Vegetos

Journal: Vegetos- An International Journal of Plant Research

Article DOI :10.5958/2229-4473.2017.00047.7
Year :2017, Volume : 30, Issue :special
First page : (132) Last page : (138)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Evaluating Microsatellite Markers for the use in Genetic Analysis of Hexaploid Wheat Varieties and Elite Lines

Phougat D.*, Singh V., Panwar I. S., Yashveer S.1

Department of Genetics & Plant Breeding, Biotechnology & Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125 004, India
1Department of Molecular Biology, Biotechnology & Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125 004, India

*Corresponding author: D Phougat, Department of Genetics & Plant Breeding, Biotechnology & Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125 004, India, E-mail: divyaphougat786@gmail.com

Genetic diversity and population structure of elite wheat (Triticum aestivum L. em. Thell) breeding lines promote effective use of genetic resources. In this study, 25 released and advanced breeding lines of wheat were analysed using 44 polymorphic SSRs. The highest number of alleles per locus was detected in the genome A as 3.14, compared to 2.68 and 2.80 for genomes B and D, respectively. The allelic polymorphism information content (PIC) value ranged from 0.10 (barc 7 and xgdm 132) to 0.86 (barc 65) with an average of 0.48. Twenty five bread wheat varieties/elite lines were grouped into two clusters using dendogram analysis. Highest similarity was observed between WH 1081 and WH 283 varieties. Aus 158854 & HD 2967 of Cluster I were the most divergent genotypes with a similarity index of 0.654followed by Tobari & WH 416 of Cluster II with a similarity index of 0.656. The result of the study indicates that the registered varieties in our country posses relatively narrow genetic variation.




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