Multi-Functional Enzyme Ability of Fungal Strain, Aspergillus niger, Screened from Dump of Pig Waste
Tyagi Sonika, Lata Suman, Sahrawat Alka1, Purushottum2
Department of Biotechnology, Meerut Institute of Engineering & Technology, Meerut-250005
1Department of Microbiology, CCS University, Meerut
2Department of Pathology & Microbiology, S V P University of Agriculture & Technology, Meerut
*Corresponding author: Sonika Tyagi, Department of Biotechnology, Meerut Institute of Engineering & Technology, Meerut-250005, Email: firstname.lastname@example.org
Microbes and fungi are the major source of industrial enzymes. In this study, thirty seven fungal strains were isolated from various locations like poultry farm and dumps of pig wastes. All the fungal isolates were examined for their capability to hydrolyse organic complex molecules such as sodium phytate, citrus pectin starch, carboxymethyl cellulose, tannic acid, casein in presence of phytase, pectinase, amylase, cellulases, tannase and protease enzymes. Isolated fungal cultures were inoculated on substrate specific solid agar plates to check the multi-functional enzymes. After incubation at 30°C, fungal cultures exhibiting enzyme activity showed zone of clearance around the colony by hydrolyzing the substrate. The fungal culture, Aspergillus niger (FN2) was isolated from pig waste, Ghaziabad (UP) which exhibited maximum phytase activity to hydrolyse sodium phytate. The fungal isolate also exhibits pectinase and amylolytic activities and is capable to hydrolyse citrus pectin and starch. No cellulase, tannase and protease activity were seen on substrate specific solid agar plates. These fungal enzymes have application in feed, food, textile, leather, paper and pharmaceutical industries.