Salicylic acid Induced changes in Biomass and Elicits Primary Metabolites under in vitro Cultured Callus of Snow Mountain Garlic
Zahoor Aanisia*, Afaque Mehjabeen, John Suchit A., Shukla Pradeep K, Misra Pragati1
Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
1Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India
*Corresponding author: Aanisia Zahoor, Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, Uttar Pradesh, India, Email: email@example.com
Snow Mountain Garlic, also called as Kashmiri garlic, found at an altitude of 6000 feet above the sea level usually in the snow covered mountains of the Himalayas. The present work embodies the effects of different concentrations of salicylic acid (SA) on accumulation and enhancement of various metabolites in callus cultures of snow mountain garlic. In order to regulate the production of metabolites, SA was supplemented as elicitor in different concentrations (0, 25, 50, 75, 100 mg/l) out of which 50 mg/l concentration was found to be most suitable for eliciting different metabolites. SA 50mg/l also caused an increase in callus biomass (8.8g) as compared to the control (6.2g) after 30 days of treatment. The elicitation effect of SA 50mg/l on different metabolites was observed after 15 days of treatment. Enhancement in total carbohydrate content was 29.62mg/g after SA treatment in comparison to control (23.30mg/g). The total protein content (22.9mg/g) was significantly increased by application of SA as compared with control (18.6 mg/g). The influence of selected concentration of SA caused a considerable increase in proline content (0.069μmol/g) of snow mountain garlic as compared to control (0.034μmol/g). These findings could be used for biotechnological production of secondary metabolites like Allicin, Allyl disulfide oxide and S-Allyl- Cysteine.