Isolation and Screening of Osmotolerant Endophytic Bacteria from Succulent and Non-Succulent Drought Tolerant plants for water stress alleviation in Cluster Bean (Cyamopsis tetrogonoloba)
Maheshwar Singh Rathi*, Sangeeta Paul, B. S. Manjunatha, Vivek Kumar1 and Ajit Varma1
Division of Microbiology, Indian Agricultural Research Institute, New Delhi
110 012, India
1Amity Institute of Microbial Technology, Amity University, Noida 201313, U. P, India
Division of Microbiology, Indian Agricultural Research Institute, New Delhi- 10012, India. Email: email@example.com
In the present investigation endophytic bacteria were isolated from succulent (Nagphani) and non-succulent (mustard) plants growing in drought prone regions of Rajasthan. Out of the sixty eight endophytic bacteria, twenty six were highly osmotolerant and in presence of 30% PEG 6000 showed 50% or higher growth as compared to that obtained under control conditions. Effect of osmotic stress on their growth was determined. Although, at concentrations higher than 30% PEG 6000, growth of most of the selected endophytes was reduced, however, these were observed to be able to grow even in presence of 50% PEG 6000. Inoculation with most of the isolates from both the sources improved fresh and dry weight of shoot, number of flowers and pods formed and dry weight of roots. On the basis of most of the parameters studied, two promising isolates were identified, which can be used as biofertilizers to improve productivity of Cluster bean in arid and semi-arid regions of the country.