Leaf Segment Size and Culture Media influence the recovery and Diversity of Fungal Endophytes isolated from Madhuca longifolia with their Enzymes Activity
Satish K Verma, Surendra K Gond1, Ashish Mishra, Vijay K Sharma, Anuj Kumar2 and Ravindra N Kharwar*
Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, India-221005 1Department of Botany, MMV, BHU, Varanasi Department of Botany, Buddha PG College, Kushinagar, India
Centre of Advanced Study in Botan y,Institute of Science, Banaras Hindu University, Varanasi, India-221005 E-mail: email@example.com
This study was performed to analyse the impact of leaf segment size and culture media on isolation and diversity of endophytic fungi from Madhuca longifolia with enzyme production potential. Total 14 taxa of endophytic fungi were recovered, dominated by Colletotrichum gloeosporioides, Corynespora and Macrophoma respectively. Reduction in leaf segment size showed increased isolation rate and significantly changed the diversity to all culture media. On potato dextrose agar 153 isolates were recovered with minimum isolation rate and diversity (1.20 isolates/cm2, Shannon index 1.43) to larger size segments (T1), followed by medium size segments (T2) (1.95 isolates/ cm2, Shannon index 1.78) and maximum to small size segments (T3) (4.50 isolates/cm2, Shannon 1.89). On malt extract agar 145 isolates were recovered in which large size segments showed minimum isolation rate and diversity (1.30 isolates/cm2, Shannon of 1.33) followed by medium size (isolation rate 1.95 isolates/cm2, Shannon of 1.55) and maximum to small size segments (isolation rate 4.0 isolates/ cm2, Shannon of 1.96). On water agar, 108 isolates were recovered with similar trends of isolation rate and diversity to large size (0.65 isolates/cm2, Shannon of 0.27) followed by medium size (isolation rate 1.40 isolates/cm2, Shannon of 1.42) and maximum to small size segments (3.35 isolates/cm2, Shannon of 1.59). This study revealed significantly that smaller leaf segment was most suitable size that gives real measures of isolation rate and diversity. Interestingly, of 24 endophytic fungi evaluated for enzyme activity, 18 were found positive to amylase, 9 to cellulase, 5 to pectinase and 14 to urease, respectively.