Plant Regeneration from Alginate encapsulated nodes of A ndrographis paniculata (Burm. F.) Wallich ex Nees. A Potential Medicinal Herb
Madhulika Singh*, Chhaya Yadav and Farha Bano1
1Department of Botany, Banaras Hindu University, Varanasi-221 005, U.P., India
Department of Biotechnology, Institute of Biosciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kanpur-208 024, U.P., India E-mail: email@example.com
Synthetic seed technology offers tremendous potential for micropropagation, conservation and transport of germplasm of plant species. A ndrographis paniculata (burm. F.) wallich ex nees. is known to possess variety of medicinal value and this study reports germination of encapsulated nodes of A . paniculata under different experimental conditions. The conversion of encapsulated node was affected by sodium alginate concentration and 3% (w/v) sodium alginate was best for encapsulation. Supplementation of medium with low concentration of BAP showed a slight increase in percent germination of encapsulated nodes. Among fructose, glucose, maltose and sucrose the best percent germination was recorded on medium having fructose while shoot growth was favoured in presence of sucrose. One-step conversion of encapsulated node was recorded in half strength growth regulator free Murashige and Skoog (MS) liquid medium. The encapsulated nodes showed capability to retain their viability after storage at 4°C. About 95% synthetic seeds converted into plantlets on half strength MS liquid medium successfully hardened and acclimatized into pots filled with sand and garden soil (3:1) mixture.