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Vegetos

Vegetos- An International Journal of Plant Research & Biotechnology

Article DOI :10.5958/2229-4473.2018.00014.9
Year :2018, Volume : 31, Issue :1
First page : (100) Last page : (103)
Print ISSN : 0970-4078. Online ISSN : 2229-4473.


RESEARCH ARTICLE
A SOCIETY FOR PLANT RESEARCH PUBLICATION

Regenerative Competence in Root explants of Medicinally important Orchid Cymbidium aloifolium: An in vitro study

Shivani Verma* and Promila Pathak

Orchid laboratory, Panjab University, Chandigarh- 160 014, India

Orchid laborator y, Panjab University, Chandigarh- 160 014, India Email: shiv.ni.verma@gmail.com

The paper reports the possibility of using root explants for micropropagating Cymbidium aloifolium. Mitra et al. (1976) medium supplemented with 2, 4-D ( 2mgl-1) induced callusing at the root tip. However, cent% explants responded via shoot bud formation within 13.25 ± 0.95 days. The neoformations organogenated subsequently. The plantlets grown on M medium supplemented with NAA (3mgl-1) and those with 4-5 leaves and a number of roots and further these were subjected to a two-step hardening procedure by subjecting them to in vitro and ex vitro hardening. These survived with 60% frequency.




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