VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

Research Articles


Volume: 34, Issue: 2, June 2021

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Views: 199

Doi: 10.1007/s42535-021-00203-3
Doi Link:
First Page: 325
Last Page: 331
Published: 25 March, 2021

Micropropagation and clonal fidelity assessment of acclimatized plantlets of Crotalaria longipes Wight & Arn. using ISSR markers


An efficient protocol for micropropagation of Crotalaria longipes Wight & Arn., an endemic and endangered Indian legume, was developed. Nodal explants cultured on Murashige and Skoog (MS) supplemented with Thidiazuron (TDZ) 1.0 mg L−1 and NAA 0.5 mg L−1 showed maximum shoot induction. The multiplied shoots were subsequently sub-cultured every 3 weeks on the same medium. The separated microshoots cultured on half-strength MS basal salts supplemented with 0.5 mg L−1 indole-3-butyric acid (IBA) showed maximum root induction. After 4 weeks of culture, the rooted shoots (> 6 cm) were planted in pots containing coco peat and garden soil (1:1) covered with plastic domes and maintained at 25 ºC for 2 weeks. After 2 weeks, the potted plants were transferred to a glasshouse, maintained at 25 °C with a relative humidity of 80%. The acclimatized plants were then successfully established in soil with an 80% survival rate. Clonal fidelity assessment of acclimatized plantlets was confirmed using inter simple sequence repeat (ISSR). All the in vitro developed plants showed monomorphic banding pattern similar to the mother plant, thus ascertaining the true-to-type nature of the in vitro raised plants.



Clonal fidelity, n Crotalaria longipesn , ISSR markers, Legume, Micropropagation


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Acknowledgements :

This research was part of the Lead Garden Project, funded by the Ministry of Environment, Forest and Climate Change (MoEF & CC), New Delhi, India. The authors are grateful to the Director, BSI, Kolkata for the constant encouragement and support.

Author Information:

Kaliamoorthy Seventhilingam
National Orchidarium and Experimental Garden, Botanical Survey of India, Southern Regional Center, Tamil Nadu, Yercaud, India
Balaji V. Kalaivanan
National Orchidarium and Experimental Garden, Botanical Survey of India, Southern Regional Center, Tamil Nadu, Yercaud, India

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