VEGETOS: An International Journal of Plant Research & Biotechnology
(Society For Plant Research)

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Doi: 10.1007/s42535-021-00284-0
Doi Link: https://doi.org/10.1007/s42535-021-00284-0
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Published: 14 August, 2021

Development of advance pearl millet lines tolerant to terminal drought stress using marker-assisted selection


Abstract:

HHB 226 is a popular, high-yielding pearl millet hybrid cultivated widely in the Northern part of India. Drought stress at post-flowering stage is one of the major constraints in realizing potential yields of this hybrid. Developing drought-tolerant pearl millet hybrid HHB 226 is priority for pearl millet breeders. Transferring the drought-tolerant quantitative trait loci (QTLs) from donor source (863 B) to male parent of HHB 226 (HBL 11) was undertaken to improve HHB 226. The drought-tolerant donor source, 863 B was backcrossed with recurrent parent (HBL 11) up to BC3F1 generation and drought-tolerant QTLs were transferred through marker-assisted backcrossing (MABC) strategy. Four polymorphic simple sequence repeat (SSR) markers linked to drought-tolerant QTLs were used to select the plants with drought-tolerant QTLs in each generation. In BC3F1 generation, four such plants were selected. Background selection was done in BC2F1 and BC3F1 generations for recovery of recurrent parent genome. Thirty-two background markers, found polymorphic out of 64 primers screened, were further used for background selection. About 80% of recurrent parent genome has been recovered in selected plants of BC3F1 generation. The results demonstrated that drought-tolerant QTLs transferred through MAS successfully improved drought tolerance in HHB 226.

Vegetos

Keywords:


Pearl millet, Drought tolerance, Marker-assisted backcrossing, QTLs, SSRs


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Acknowledgements :



Asha is thankful to CCS Haryana Agricultural University, Hisar (India), for providing financial assistance in the form of Merit scholarship.


Author Information:



Rani, Asha
Department of Molecular Biology, Biotechnology, and Bioinformatics, CCS Haryana Agricultural University, Hisar, India

Taunk, Jyoti
Department of Molecular Biology, Biotechnology, and Bioinformatics, CCS Haryana Agricultural University, Hisar, India


Jangra, Sumit
Department of Molecular Biology, Biotechnology, and Bioinformatics, CCS Haryana Agricultural University, Hisar, India

Yadav, Ram C.
Department of Molecular Biology, Biotechnology, and Bioinformatics, CCS Haryana Agricultural University, Hisar, India

rcyadavbiotech@yahoo.com Yadav, Neelam R.
Department of Molecular Biology, Biotechnology, and Bioinformatics, CCS Haryana Agricultural University, Hisar, India

Yadav, Devvart
Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, India

Yadav, H. P.
Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar, India




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