Establishment of a correlation between pistil size and female gametophyte developmental stages in apomictic and sexual lines of Cenchrus americanus (L.) Morrone

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Singh, Heisnam Dinesh, Sharma, Monika, Mohapatra, Priyanka, Goel, Shailendra, Jagannath, Arun, Agarwal, Manu


Research Articles | Published: 17 February, 2022

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00340-3
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Last Page: 0
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Keywords: Apomixis, Breeding, Female gametophyte, Pistil, Reproduction


Abstract


Apomixis is a unique reproductive strategy that leads to seed production without reductional division and gametic fusion. In order to unravel the regulatory components unique to apomictic lines, apomixis often requires a comparative study between apomictic and its sexual counterpart. Such studies require staging and harvesting of the pistil, which are the apparent choice of tissue for understanding the expression of apomictic trait. The collection and staging of the female tissue require a lot of effort and time, due to which the whole inflorescence is often used instead of harvesting individual pistils. The present study attempts to simplify the procedure of pistil collection by correlating its length with female developmental stages between sexual and apomictic lines of pearl millet. The female development stages in the pistil were confirmed through Differential Interference Contrast microscopy, providing a reliable method for the collection of tissue, saving time and effort. The tissue was kept in liquid nitrogen during collection and later stored at − 80 °C up to a year. The tissue provided excellent quality RNA, which can be used for comparative analysis between sexual and apomictic lines.

Apomixis, Breeding, Female gametophyte, Pistil, Reproduction


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Acknowledgements


This research was supported by the Department of Biotechnology (DBT) grant by the Government of India (Sanction No. BT/PR10696/AGR/36/589/2008). We are also thankful to the University of Delhi, Delhi, India, for IOE grant (Sanction No. IoE/2021/12/FRP). Heisnam Dinesh Singh and Monika Sharma are thankful to Council of Scientific and Industrial Research (CSIR), India, for their Research fellowships. Priyanka Mohapatra is thankful for the (INSPIRE) fellowship provided by the Department of Science and Technology.


Author Information


Singh, Heisnam Dinesh
Department of Botany, University of Delhi, Delhi, India

Sharma, Monika
Department of Botany, University of Delhi, Delhi, India


Mohapatra, Priyanka
Department of Botany, University of Delhi, Delhi, India

Goel, Shailendra
Department of Botany, University of Delhi, Delhi, India

shailendragoel@gmail.com Jagannath, Arun
Department of Botany, University of Delhi, Delhi, India

Agarwal, Manu
Department of Botany, University of Delhi, Delhi, India